anti-Human SIRT1 antibody for Immunohistochemistry

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Reactivity
Human
305
139
71
21
14
11
8
7
7
7
5
4
4
3
2
2
2
1
Host
Mouse
207
85
26
6
3
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
17
14
10
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
254
129
87
66
60
50
40
40
36
7
6
5
4
2
2
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN969396
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.754329 ABIN3198318 ChIP IC IF IHC IP WB Rabbit Log in to see Polyclonal
9.754329 ABIN2470013 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
9.754329 ABIN2471078 IF IHC ELISA WB Mouse IgG1 kappa AA 456-555 Log in to see 7B7
9.754329 ABIN1874784 ChIP IF IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal
9.754329 ABIN4353899 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-550, Internal Region Log in to see Polyclonal
9.754329 ABIN3032583 IF IHC ELISA WB Rabbit Ig Fraction AA 566-601 Log in to see Polyclonal
9.754329 ABIN2403466 ChIP IF IP IHC WB Rabbit IgG Log in to see Polyclonal
9.754329 ABIN1846081 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
1 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
1 ABIN3186962 ELISA IHC WB Rabbit IgG Tyr631 Log in to see Polyclonal
1 ABIN4353903 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
1 ABIN1525672 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1450758 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 3
1 ABIN3020731 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1874783 IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1176381 ICC IHC IP WB Rabbit IgG AA 244-498 Log in to see Polyclonal
1 ABIN4880459 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 1F3
1 ABIN625834 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN1824781 IP IHC WB Rabbit IgG AA 702-714 Log in to see Polyclonal
1 ABIN1457379 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-SIRT1 antibody for Immunohistochemistry

Similar anti-SIRT1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 5 Antibodies
ELISA 112 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 41 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 48 Antibodies
Immunofluorescence (IF) 65 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 83 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 234 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Reactivity Human
(305), (139), (71), (21), (14), (11), (8), (7), (7), (7), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(207), (85), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(17), (14), (10), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(254), (129), (87), (66), (60), (50), (40), (40), (36), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN969396) Images
Purification Ascites
Immunogen Purified recombinant fragment of human SIRT1 expressed in E. Coli.
Clone 1F3
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN969396) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molecular Weight 120 kDa
Gene ID 23411
HGNC 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling ProductDetails: References for anti-SIRT1 antibody (ABIN969396) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details ProductDetails: References for anti-SIRT1 antibody (ABIN969396) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-SIRT1 antibody (ABIN969396)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, 2009

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN969396) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 1: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (ABIN969396) Figure 4: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), ...