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anti-Human YAP1 antibody for Immunofluorescence

Recommended YAP1 Antibody (supplied by: Log in to see )

Antigen
Yes-Associated Protein 1 (YAP1) Antibodies
  • AI325207
  • cb194
  • HCCA1
  • HCCA2
  • sb:cb194
  • si:ch211-181p1.5
  • si:dkey-3b8.3
  • wu:fc18c04
  • xyap
  • yap
  • Yap
  • YAP
  • YAP1
  • yap2
  • YAP2
  • Yap65
  • yap65
  • YAP65
  • yki
  • Yki
  • YKI
  • Yorkie
  • YY1AP
  • zgc:158380
Epitope
AA 53-161, partial
22
20
17
17
14
13
13
13
12
11
6
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
195
70
61
5
2
2
2
2
2
1
1
Host
Mouse
147
38
9
1
Clonality
Monoclonal
Conjugate
This YAP1 antibody is un-conjugated
8
7
7
6
6
6
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
146
91
48
34
33
22
16
16
16
10
6
3
1
Supplier
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Catalog No. ABIN564526
$ 430.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31104264 ABIN614702 EIA IF IHC (p) WB Mouse IgG2a AA 53-162 Log in to see 2F12
0.31104264 ABIN2707258 IC IF IP IHC WB Rabbit Center Log in to see Polyclonal
0.31104264 ABIN614703 EIA IF IHC (p) WB Mouse IgG2a AA 53-162 Log in to see 2H1
0.31104264 ABIN523813 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 53-161, partial Log in to see 2H1
0.31104264 ABIN523812 IF ELISA WB Mouse IgG2a kappa AA 53-161, partial Log in to see 2C2
0.0010891865 ABIN395709 IF WB Mouse IgG2a kappa AA 53-162 Log in to see 2H1 5
0.0010891865 ABIN395579 IF WB Mouse IgG2a kappa AA 53-162 Log in to see 2F12 5
0.0010891865 ABIN272194 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010891865 ABIN256928 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1674Y
0.0010891865 ABIN1723562 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 53-162 Log in to see 2F12
0.0010891865 ABIN1723563 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 53-162 Log in to see 2H1
0.0010891865 ABIN2471055 IF IHC ELISA WB Mouse IgG2a kappa AA 53-162, partial Log in to see 2F12
0.0010891865 ABIN2471056 IF IHC ELISA WB Mouse IgG2a kappa AA 53-162, partial Log in to see 2H1
0.0010891865 ABIN1515202 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010891865 ABIN2892115 IF IHC WB Rabbit Gln295 Log in to see Polyclonal
0.0010891865 ABIN258560 ChIP ICC IF IHC (p) IP IHC WB Rabbit Log in to see Polyclonal
0.0010891865 ABIN574419 IF IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see 2F12
0.0010891865 ABIN3187513 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0010891865 ABIN1740894 ICC IF IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0010891865 ABIN574420 IF IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see 2H1

Top referenced anti-YAP1 antibody for Immunofluorescence

Similar anti-YAP1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
Dot Blot (DB) 16 Antibodies
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen Yes-Associated Protein 1 (YAP1) Antibodies
Epitope AA 53-161, partial
(22), (20), (17), (17), (14), (13), (13), (13), (12), (11), (6), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(195), (70), (61), (5), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(147), (38), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This YAP1 antibody is un-conjugated
(8), (7), (7), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(146), (91), (48), (34), (33), (22), (16), (16), (16), (10), (6), (3), (1)
Pubmed 14 references available
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Product Details anti-YAP1 Antibody

Target Details YAP1 Application Details Handling References for anti-YAP1 Antibody (ABIN564526) Images
Cross-Reactivity Human
Immunogen YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: QIVHVRGDSE TDLEALFNAV MNPKTANVPQ TVPMRLRKLP DSFFKPPEPK SHSRQASTDA GTAGALTPQH VRAHSSPASL QLGAVSPGTL TPTGVVSGPA ATPTAQHLR
Clone 2F12
Isotype IgG2a kappa

Target Details YAP1

Product Details anti-YAP1 Antibody Application Details Handling References for anti-YAP1 Antibody (ABIN564526) Images back to top
Antigen
Alternative Name YAP1 (YAP1 Antibody Abstract)
Background Full Gene Name: Yes-associated protein 1, 65 kDa
Synonyms: YAP,YAP2,YAP65,YKI
Gene ID 10413
NCBI Accession NP_006097, NM_006106
Pathways MAPK Signaling

Application Details

Product Details anti-YAP1 Antibody Target Details YAP1 Handling References for anti-YAP1 Antibody (ABIN564526) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only

Handling

Product Details anti-YAP1 Antibody Target Details YAP1 Application Details References for anti-YAP1 Antibody (ABIN564526) Images back to top
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-YAP1 Antibody (ABIN564526)

Product Details anti-YAP1 Antibody Target Details YAP1 Application Details Handling Images back to top
Product cited in:

Yang, Zhang, Chen, Chen, Dong: "Oncoprotein YAP regulates the spindle checkpoint activation in a mitotic phosphorylation-dependent manner through up-regulation of BubR1." in: The Journal of biological chemistry, Vol. 290, Issue 10, pp. 6191-202, 2015

Shao, Zhai, Del Re, Sciarretta, Yabuta, Nojima, Lim, Pan, Sadoshima: "A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response." in: Nature communications, Vol. 5, pp. 3315, 2014

Adler, Heller, Bringman, Ranahan, Cocklin, Goebl, Oh, Lim, Ingham, Wells: "Amot130 adapts atrophin-1 interacting protein 4 to inhibit yes-associated protein signaling and cell growth." in: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 15181-93, 2013

Das, Tanigawa, Karner, Xin, Lum, Chen, Olson, Perantoni, Carroll: "Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation." in: Nature cell biology, Vol. 15, Issue 9, pp. 1035-44, 2013

Yang, Zhang, Liu, Chong, Ding, Chen, Dong: "CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is essential for neoplastic transformation." in: Cancer research, Vol. 73, Issue 22, pp. 6722-33, 2013

Adler, Johnson, Heller, Bringman, Ranahan, Conwell, Sun, Hudmon, Wells: "Serum deprivation inhibits the transcriptional co-activator YAP and cell growth via phosphorylation of the 130-kDa isoform of Angiomotin by the LATS1/2 protein kinases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 43, pp. 17368-73, 2013

Skouloudaki, Walz: "YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35735, 2012

Hirate, Cockburn, Rossant, Sasaki: "Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation mouse embryos." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 50, pp. E3389-90; author reply E3391-2, 2012

Murakami, Mizuno, Taniguchi, Fujii, Ishiguro, Fukui, Akatsuka, Horio, Hida, Kondo, Toyokuni, Osada, Sekido: "LATS2 is a tumor suppressor gene of malignant mesothelioma." in: Cancer research, Vol. 71, Issue 3, pp. 873-83, 2011

Leivonen, Rokka, Ostling, Kohonen, Corthals, Kallioniemi, Perälä: "Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 7, pp. M110.005322, 2011

Sekido: "Inactivation of Merlin in malignant mesothelioma cells and the Hippo signaling cascade dysregulation." in: Pathology international, Vol. 61, Issue 6, pp. 331-44, 2011

Wada, Itoga, Okano, Yonemura, Sasaki: "Hippo pathway regulation by cell morphology and stress fibers." in: Development (Cambridge, England), Vol. 138, Issue 18, pp. 3907-14, 2011

Tomlinson, Gudmundsdottir, Luong, Leung, Knebel, Basu: "JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis." in: Cell death & disease, Vol. 1, pp. e29, 2011

Lau, Murray, Houshmandi, Xu, Gutmann, Yu: "Merlin is a potent inhibitor of glioma growth." in: Cancer research, Vol. 68, Issue 14, pp. 5733-42, 2008

Images

Product Details anti-YAP1 Antibody Target Details YAP1 Application Details Handling References for anti-YAP1 Antibody (ABIN564526) back to top
Supplier Images
Immunostaining (ISt) image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Immunoperoxidase of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded h...
Western Blotting (WB) image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) YAP1 monoclonal antibody (M01), clone 2F12. Western Blot analysis of YAP1 expression ...
Immunofluorescence (IF) image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell. [antibody concentrati...
ELISA image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Detection limit for recombinant GST tagged YAP1 is approximately 0.03ng/ml as a captu...
Western Blotting (WB) image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Western Blot analysis of YAP1 expression in transfected 293T cell line by YAP1 monocl...
Western Blotting (WB) image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Immunoprecipitation of YAP1 transfected lysate using anti-YAP1 monoclonal antibody an...
 image for anti-YAP1 antibody (Yes-Associated Protein 1) (AA 53-161) (ABIN564526) Western Blot detection against Immunogen (37.73 KDa) .