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anti-Human Kinesin Heavy Chain Member 2A antibody for Western Blotting

Recommended Kinesin Heavy Chain Member 2A Antibody (supplied by: Log in to see )

Antigen
Kinesin Heavy Chain Member 2A (KIF2A) Antibodies
  • C530030B14Rik
  • fj55b02
  • HK2
  • Kif2
  • KIF2
  • kif2-a
  • kinesin-II
  • Kns2
  • kns2
  • M-kinesin
  • wu:fj55b02
Epitope
N-Term
9
6
3
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
40
16
15
2
2
2
2
2
1
Host
Rabbit
28
12
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
33
19
14
7
7
5
2
1
1
1
Supplier
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Catalog No. ABIN2778183
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2778182 WB Rabbit Middle Region Log in to see Polyclonal 1
0.13341247 ABIN2778232 WB Rabbit C-Term Log in to see Polyclonal 1
0.13341247 ABIN2458064 ELISA WB Rabbit Log in to see Polyclonal
0.13341247 ABIN634106 WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN634105 WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN634112 WB Rabbit Middle Region Log in to see Polyclonal
0.13341247 ABIN2423701 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN268909 ICC IF IP SimWes WB Rabbit N-Term Log in to see Polyclonal 9
0.0011728023 ABIN561592 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-679, full length Log in to see 2D4 1
0.0011728023 ABIN324346 WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011728023 ABIN467927 WB Rabbit Internal Region Log in to see Polyclonal
0.0011728023 ABIN958997 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN395518 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-680 Log in to see 2D4
0.0011728023 ABIN598770 ELISA WB Mouse IgG1, kappa Log in to see 2D4
0.0011728023 ABIN2742382 WB Rabbit IgG AA 1-11 Log in to see Polyclonal
0.0011728023 ABIN3198174 IC IF WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1908105 IHC ELISA WB PE Mouse IgG1 AA 1-680 Log in to see
0.0011728023 ABIN1908102 IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 AA 1-680 Log in to see
0.0011728023 ABIN1908106 IHC ELISA WB Biotin Mouse IgG1 AA 1-680 Log in to see
0.0011728023 ABIN1908104 IHC ELISA WB FITC Mouse IgG1 AA 1-680 Log in to see

Top referenced anti-Kinesin Heavy Chain Member 2A antibody for Western Blotting

Similar anti-Kinesin Heavy Chain Member 2A Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 13 Antibodies 34 Antibodies 14 Antibodies 3 Antibodies
Immunoprecipitation (IP) 7 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 15 Antibodies 6 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 5 Antibodies 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Kinesin Heavy Chain Member 2A (KIF2A) Antibodies
Epitope N-Term
(9), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(40), (16), (15), (2), (2), (2), (2), (2), (1)
Host Rabbit
(28), (12)
Clonality
Polyclonal
Conjugate Un-conjugated
(1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(33), (19), (14), (7), (7), (5), (2), (1), (1), (1)
Pubmed 1 reference available
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Product details

Target details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN978956
Sequence IEPSPETPPP PASSAKVNKI VKNRRTVASI KNDPPSRDNR VVGSARARPS
Predicted Reactivity Human : 100 %, Dog : 90 %, Guinea pig : 90 %, Rabbit : 90 %, Rat : 90 %, Bovine : 80 %, Horse : 80 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against KIF2A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human KIF2A

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name KIF2A (KIF2A Antibody Abstract)
Background KIF2A belongs to the kinesin-like protein family, MCAK/KIF2 subfamily. It contains 1 kinesin-motor domain. KIF2A plus end-directed microtubule-dependent motor required for normal brain development. It may regulate microtubule dynamics during axonal growth. KIF2A is implicated in formation of bipolar mitotic spindles. It has microtubule depolymerization activity. Hela cells lacking KIF2A show asymmetric or monopolar mitotic spindles. Osteosarcoma cells (U2OS) lacking KIF2A or KIF2B show disorganised or monopolar mitotic spindles.Kinesins, such as KIF2, are microtubule-associated motor proteins. For background information on kinesins, see MIM 148760.
Molecular Weight 80 kDa
Gene ID 3796
NCBI Accession NP_004511, NM_004520
UniProt O00139
Research Area Cytoskeleton
Pathways Microtubule Dynamics

Application Details

Product details Target details Handling References Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 706 AA

Restrictions For Research Use only

Handling

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Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Jang, Wong, Coppinger, Seki, Yates, Fang: "DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement." in: The Journal of cell biology, Vol. 181, Issue 2, pp. 255-67, 2008

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Kinesin Heavy Chain Member 2A (KIF2A) (N-Term) antibody (ABIN2778183) WB Suggested Anti-KIF2A Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-Kinesin Heavy Chain Member 2A (KIF2A) (N-Term) antibody (ABIN2778183) Immunohistochemistry with Thymus tissue at an antibody concentration of 5µg/ml using ...