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anti-Dog (Canine) ROCK2 antibody for Immunohistochemistry

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • B230113H15Rik
  • mKIAA0619
  • Rho-kinase
  • Rock-II
  • ROCK-II
  • rock2
  • ROCK2
  • Rock2m
  • rocka-a
  • ROK
  • rok2
  • ROKalpha
  • xROK
Epitope
AA 567-718
15
14
13
13
6
6
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
158
90
88
4
2
2
2
1
1
1
1
Host
Mouse
138
20
1
Clonality
Monoclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
118
45
39
34
29
23
19
12
3
1
1
Supplier
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Catalog No. ABIN968071
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011344259 ABIN968070 IF IP IHC WB Mouse IgG1 AA 567-718 Log in to see 21-ROCK 5

Top referenced anti-ROCK2 antibody for Immunohistochemistry

Similar anti-ROCK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 19 Antibodies 19 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 10 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 21 Antibodies
Immunoprecipitation (IP) 8 Antibodies 10 Antibodies
Western Blotting (WB) 54 Antibodies 56 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope AA 567-718
(15), (14), (13), (13), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(158), (90), (88), (4), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(138), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK2 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(118), (45), (39), (34), (29), (23), (19), (12), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN968071) Images
Cross-Reactivity Mouse (Murine), Dog (Canine), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ROKalpha aa. 567-718
Clone 21-ROCK
Isotype IgG1

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling References for anti-ROCK2 Antibody (ABIN968071) Images back to top
Antigen
Alternative Name ROCK-II (ROCK2 Antibody Abstract)
Background Activity of the GTP-binding proteins is regulated by GAPs, that accelerate binding, and GTPases, that enhance the rate of GTP hydrolysis (Ras, Rho, cdc42Hs, and Rac). The isoforms of Rho, a small GTP-binding protein, regulate cellular processes such as the formation of the stress fibers, lamelipodia, and filopodia. ROKalpha (RhoA-binding kinase) is a Ser/Thr protein kinase that interects with the GTP-binding form of RhoA. Like RhoA, ROKalpha localizes at the cellular margins and colocalizes with acti filaments. The ROKalpha gene encodes a protein of 1302 amino acids with homology to the human myotonic kinase. Although ROKalpha binds to GTP-RhoA, it is not yet clear whether this interaction induces the kinase activity of ROKalpha.
Synonyms: ROKalpha
Molecular Weight 180 kDa
Pathways Microtubule Dynamics, WNT Signaling

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling References for anti-ROCK2 Antibody (ABIN968071) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details References for anti-ROCK2 Antibody (ABIN968071) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-ROCK2 Antibody (ABIN968071)

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling Images back to top
Product cited in:

Begum, Sandu, Ito, Lohmann, Smolenski: "Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6214-22, 2002

Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, 2002

Adachi, Vita, Sannohe, Stafford, Alam, Kayaba, Chihara: "The functional role of rho and rho-associated coiled-coil forming protein kinase in eotaxin signaling of eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4609-15, 2001

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, 2001

Leung, Manser, Tan, Lim: "A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29051-4, 1996

Images

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Handling References for anti-ROCK2 Antibody (ABIN968071) back to top
Supplier Images
Western Blotting (WB) image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (AA 567-718) (ABIN968071) Western blot analysis of ROCK-II on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, l...
 image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (AA 567-718) (ABIN968071) MCF7
 image for anti-ROCK2 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 2) (AA 567-718) (ABIN968071) anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 567-718) antibody (Image 3)