anti-Mouse (Murine) ROCK2 antibody for Enzyme Immunoassay

Recommended ROCK2 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
  • rocka-a
  • xROK
  • rock2
  • rok2
  • ROCK2
  • B230113H15Rik
  • ROKalpha
  • Rho-kinase
  • Rock-II
  • Rock2m
  • mKIAA0619
  • ROCK-II
  • ROK
  • Rho-associated, coiled-coil containing protein kinase 2
  • rho-associated, coiled-coil containing protein kinase 2a
  • Rho-associated coiled-coil containing protein kinase 2
  • Rho-associated coiled-coil containing protein kinase 2-like
  • rock2
  • rock2a
  • ROCK2
  • Rock2
  • LOC100346837
Epitope
pTyr256
16
15
15
12
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
170
117
112
8
5
4
3
3
2
2
1
1
1
Host
Rabbit
149
21
1
Clonality
Polyclonal
Conjugate
This ROCK2 antibody is un-conjugated
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
126
45
40
38
35
28
25
11
3
2
2
Supplier
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Supplier Product No.
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Catalog No. ABIN5551814
$ 411.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554279 EIA WB Rabbit Center Log in to see Polyclonal

Similar anti-ROCK2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Chicken Fruit Fly (Drosophila melanogaster) Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 126 Antibodies 68 Antibodies 73 Antibodies 6 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 28 Antibodies 16 Antibodies 20 Antibodies 2 Antibodies 2 Antibodies

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies
Epitope pTyr256
(16), (15), (15), (12), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(170), (117), (112), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(149), (21), (1)
Clonality
Polyclonal
Conjugate This ROCK2 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(126), (45), (40), (38), (35), (28), (25), (11), (3), (2), (2)
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Product Details anti-ROCK2 Antibody

Target Details ROCK2 Application Details Handling Images
Specificity This phospho-specific polyclonal antibody reacts with Human ROCK-2 pTyr256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Chicken, Canine, Bovine, Xenopus, Drosophila.
Species reactivity (tested):Human.
Characteristics Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)
Purification Affinity Chromatography.
Immunogen Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2
Isotype IgG

Target Details ROCK2

Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ROCK2 (ROCK2 Antibody Abstract)
Background Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or Rh °C, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2
Gene ID 9475
NCBI Accession NP_004841
UniProt O75116
Research Area Apoptosis/Necrosis, Cancer
Pathways Microtubule Dynamics, WNT Signaling, Tube Formation

Application Details

Product Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
Application Notes ELISA: 1/2,000-1/10,000. Western Blot: 1/200-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ROCK2 Antibody Target Details ROCK2 Application Details Images back to top
Concentration 1.1 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.