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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||126 Antibodies||68 Antibodies||73 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||28 Antibodies||16 Antibodies||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies||27 Antibodies||27 Antibodies|
|Immunohistochemistry (IHC)||35 Antibodies||20 Antibodies||20 Antibodies|
|Antigen||rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) Antibodies|
|Epitope||AA 652-923 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ROCK2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-ROCK2 AntibodyTarget Details ROCK2 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Rho-associated, coiled-coil containing protein kinase 2
Protein Name: Rho-associated protein kinase 2
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.|
Target Details ROCK2Product Details anti-ROCK2 Antibody Application Details Handling Images back to top
|Alternative Name||ROCK2 (ROCK2 Antibody Abstract)|
Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.
Synonyms: coiled-coil-containing protein kinase 2 antibody|KIAA0619 antibody|p164 ROCK 2 antibody|p164 ROCK-2 antibody|Rho associated coiled coil containing protein kinase 2 antibody|Rho associated protein kinase 2 antibody|Rho associated, coiled coil containing protein kinase II antibody|Rho kinase 2 antibody|Rho-associated antibody|Rho-associated protein kinase 2 antibody|ROCK 2 antibody|Rock II antibody|Rock2 antibody|ROCK2_HUMAN antibody|Rock2m antibody|ROK alpha antibody|ROKalpha antibody
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Microtubule Dynamics, WNT Signaling, Tube Formation|
Application DetailsProduct Details anti-ROCK2 Antibody Target Details ROCK2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ROCK2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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