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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||17 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA||15 Antibodies||2 Antibodies||2 Antibodies|
|Antigen||Spastin (SPAST) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus), Human Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Spastin AntibodyTarget Details Spastin Application Details Handling Images
|Specificity||Specific for Spastin.|
|Immunogen||A synthetic peptide fromc-terminal region of mouse Spastin (Spastic paraplegia 4 protein) conjugated to an immunogenic carrier protein was used as the antigen.|
Target Details SpastinProduct Details anti-Spastin Antibody Application Details Handling Images back to top
|Alternative Name||Spastin (SPAST Antibody Abstract)|
ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. May also play a role in axon growth and the formation of axonal branches.
Subcellular location: Membrane, Single-pass membrane protein. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton. Cytoplasm › perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm › cytoskeleton › spindle. Note: Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form.
Tissue specificity: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord. Also known as: SPAST, Spastic paraplegia 4 protein, ADPSP, FSP2, SPG4.
|Research Area||Signaling, Metabolism, Cytoskeleton|
Application DetailsProduct Details anti-Spastin Antibody Target Details Spastin Handling Images back to top
A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Spastin Antibody Target Details Spastin Application Details Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|