|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Chicken||Yeast||Pig (Porcine)||Cow (Bovine)||Gerbil||Frog|
|Immunofluorescence (fixed cells) (IF/ICC)||15 Antibodies||9 Antibodies||11 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Simple Western (SimWes)||3 Antibodies||3 Antibodies||3 Antibodies||3 Antibodies||3 Antibodies||1 Antibodies|
|Western Blotting (WB)||67 Antibodies||219 Antibodies||300 Antibodies||203 Antibodies||66 Antibodies||2 Antibodies||58 Antibodies||7 Antibodies|
|Antigen||Tubulin, beta (TUBB) Antibodies|
|Epitope||AA 416-430 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Frog, Fungi (Fusarium), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sea Urchin, Yeast Alternatives|
|Conjugate||This TUBB antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-TUBB AntibodyTarget Details TUBB Application Details Handling Images
|Specificity||This antibody reacts to Tubulin beta.|
Species reactivity (expected):Bovine, Porcine, Rat, Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, Fungi, Sea Urchin, Myxanoeba, and Plasmodium.
Species reactivity (tested):Human.
|Immunogen||A synthetic peptide derived from aa 416-430 of human tubulin beta|
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|Alternative Name||TUBB / TUBB5 (TUBB Antibody Abstract)|
|Background||Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. This antibody does not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Ab-3 does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.Synonyms: Tubulin beta chain, Tubulin beta-5 chain|
|Gene ID||203068, 9606|
|Pathways||Microtubule Dynamics, M Phase|
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Western Blot. Immunoprecipitation. Immunohistochemistry on paraffin sections: 1/200 for 10 min at RT. Positive Control: MAD109 cell. Skin or lung
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||10 mM PBS, pH 7.4 containing 1 % BSA as stabilizer and 0,09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!|
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