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anti-Human TUBB antibody for Immunofluorescence

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Epitope
N-Term
35
27
13
13
11
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
245
128
128
33
25
17
14
13
13
11
10
9
8
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
159
137
2
1
Clonality
Monoclonal
Conjugate
This TUBB antibody is un-conjugated
10
5
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
247
91
89
75
58
47
23
18
10
7
5
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1995415
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3157438 ABIN2856496 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.3157438 ABIN2704532 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.3157438 ABIN2854998 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.3157438 ABIN933080 IF ELISA WB Mouse IgG2b kappa AA 1-445 Log in to see AT5B2
0.3157438 ABIN2498114 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.3157438 ABIN265866 ICC IF WB Mouse IgG1 Log in to see 2-28-33
0.3157438 ABIN2504336 IF IHC WB Mouse Log in to see 87CT59-3-7
0.0010826217 ABIN387758 IF IHC (p) WB Mouse IgG2b Log in to see 87CT59-3-7 5
0.0010826217 ABIN1109367 IF WB Mouse IgG1 Log in to see A10 1
0.0010826217 ABIN238519 EIA IHC (fro) IF WB Mouse IgM Log in to see TU-13 3
0.0010826217 ABIN1558194 IF WB Mouse IgG1 Log in to see 1E1-E8-H4
0.0010826217 ABIN372497 IF IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
0.0010826217 ABIN614488 EIA IF IHC (p) WB Mouse IgG3 Log in to see n-a
0.0010826217 ABIN1501915 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0010826217 ABIN962117 IF IHC (p) ELISA WB Mouse IgG2b, kappa Log in to see AT5B2
0.0010826217 ABIN305890 ICC IF IHC (p) Alexa Fluor 555 Rabbit IgG N-Term Log in to see
0.0010826217 ABIN1502466 IF IHC (p) WB Mouse IgG3 Log in to see TBN06
0.0010826217 ABIN269949 ICC IF WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal 4
0.0010826217 ABIN462130 IF IHC (p) WB Mouse IgG3 Log in to see
0.0010826217 ABIN2470642 ICC IF IHC Rabbit IgG N-Term Log in to see

Top referenced anti-TUBB antibody for Immunofluorescence

Similar anti-TUBB Antibodies

Application / Reactivity Human
BioImaging (BI) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 80 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 34 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 77 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Immunostaining (ISt) 1 Antibodies
Western Blotting (WB) 209 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Epitope N-Term
(35), (27), (13), (13), (11), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(245), (128), (128), (33), (25), (17), (14), (13), (13), (11), (10), (9), (8), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(159), (137), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB antibody is un-conjugated
(10), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(247), (91), (89), (75), (58), (47), (23), (18), (10), (7), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN1995415) Images
Specificity Human Beta-Tubulin
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Immunogen A synthetic peptide corresponding to the N-terminus of the human beta- tubulin
Clone 5
Isotype IgG2b

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN1995415) Images back to top
Antigen
Alternative Name beta-Tubulin (TUBB Antibody Abstract)
Background Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus "housekeeping genes" are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti- COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN1995415) Images back to top
Application Notes Western blot - This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Beta-Tubulin in WB. Immunochemistry - ICC/IF: 5-20 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN1995415) Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

References for anti-TUBB Antibody (ABIN1995415)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Product cited in:

Li, Chen, Cheng, Zhao, Zhao, Xu: "Amyloid precursor-like protein 2 C-terminal fragments upregulate S100A9 gene and protein expression in BV2 cells." in: Neural regeneration research, Vol. 9, Issue 21, pp. 1923-8, 2015

Images

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN1995415) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TUBB antibody (Tubulin, beta) (N-Term) (ABIN1995415) Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed wit...
Western Blotting (WB) image for anti-TUBB antibody (Tubulin, beta) (N-Term) (ABIN1995415) anti-Tubulin, beta (TUBB) (N-Term) antibody (Image 2)