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anti-Pig (Porcine) TUBB antibody for Immunocytochemistry

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • 1t
  • 2t
  • 3t
  • 4t
  • 143391_i_at
  • AA408537
  • AI325297
  • AI596182
  • B1t
  • B2t
  • B3t
  • B4t
  • B130022C14Rik
  • beta
  • beta(2)Tu
  • beta(2)Tub
  • beta-5
  • beta-tub
  • beta-Tub
  • beta-Tub6D
  • beta-Tub56D
  • beta-Tub60D
  • beta-Tub85D
  • beta-tub85D
  • beta-Tub97EF
  • beta-tubulin56D
  • beta1
  • beta1-tub
  • beta1-Tubulin
  • beta1t
  • beta1tub
  • beta1Tub
  • BETA2
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3t
  • beta3TUB
  • beta3Tub
  • beta4
  • beta4t
  • beta56D
  • BETA 56D
  • beta60C
  • BETA 60D
  • BETA 85D
  • beta85D
  • beta97F
  • BETA 98B
  • betaTub
  • betatub(56D)
  • betaTub1
  • betaTub2
  • betaTub3
  • betaTub4
  • betaTub60C
  • betatub60D
  • betaTub98BC
  • betaTub98C
  • beta[[1]]-tubulin
  • beta[[1]] tubulin
  • beta[[2]]-tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • beta[[3]] tubulin
  • bmtub1
  • bmtub2
  • bmtub3
  • bmtub4
  • CG3401
  • CG4869
  • CG9277
  • CG9359
  • D.m.BETA-60D
  • D.m.BETA-85D
  • Dmbeta1
  • Dmbeta2
  • Dmbeta3
  • Dmbeta4
  • Dmel\\CG3401
  • Dmel\\CG4869
  • Dmel\\CG9277
  • Dmel\\CG9359
  • DTB1
  • DTB2
  • DTB3
  • DTB4
  • LOC100101153
  • M(beta)4
  • M(beta)5
  • M40
  • m40
  • ms(3)KK[D]
  • OK/SW-cl.56
  • ok/sw-cl.56
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • T
  • Tub
  • Tub1
  • Tub2
  • Tub3
  • Tub4
  • tub5
  • Tub60D
  • Tubb
  • TUBB
  • TUBB1
  • tubb1
  • TUBB2A
  • tubb2a
  • TUBB2B
  • tubB3
  • tubb4
  • Tubb4
  • tubb5
  • TUBB5
  • tubulin
  • Tubulin
  • XLOT
Epitope
N-Term
35
27
13
13
11
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Mouse (Murine), Plant
245
128
127
32
25
17
14
13
13
11
10
9
8
7
6
5
4
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
159
137
2
1
Clonality
Monoclonal
Conjugate
This TUBB antibody is un-conjugated
10
5
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
247
91
89
76
58
46
23
18
9
7
5
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN192340
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009987548 ABIN239815 ICC IHC (p) WB Mouse IgM Log in to see TU-06
0.0009987548 ABIN2469988 ICC IHC WB Mouse IgM AA 81-95 Log in to see TU-06
0.0009987548 ABIN269949 ICC IF WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal 4
0.0009987548 ABIN207094 ICC IHC (fro) ELISA WB Mouse IgM N-Term Log in to see TU-13
0.0009987548 ABIN207085 ICC WB Mouse IgM C-Term Log in to see TU-12
0.0009987548 ABIN352922 ICC WB Mouse IgM AA 345-430 Log in to see
0.0009987548 ABIN352925 ICC IP ELISA WB Mouse IgM N-Term Log in to see
0.0009987548 ABIN1724148 ICC IHC (p) WB Mouse IgM Log in to see TU-06
0.0009987548 ABIN481001 ICC IHC ELISA WB Mouse IgM N-Term Log in to see
0.0009987548 ABIN1169288 ICC ELISA WB Human IgG2 Log in to see S11B
0.0009987548 ABIN3199337 ICC IHC (fro) WB Mouse IgM Log in to see TU-13
0.0009987548 ABIN2738558 ICC IHC (p) Mouse IgM Log in to see TU-06
0.0009987548 ABIN3040172 ICC IF IHC WB Mouse IgM Log in to see
0.0009987548 ABIN2793956 ICC IHC (p) IHC WB Mouse IgM Log in to see TU-06

Top referenced anti-TUBB antibody for Immunocytochemistry

Similar anti-TUBB Antibodies

Application / Reactivity Pig (Porcine) Mouse (Murine) Human Plant
Western Blotting (WB) 36 Antibodies 107 Antibodies 209 Antibodies 6 Antibodies
Immunostaining (ISt) 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 6 Antibodies 14 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 50 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 45 Antibodies 77 Antibodies 6 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies

General

Antigen Tubulin, beta (TUBB) Antibodies
Epitope N-Term
(35), (27), (13), (13), (11), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Pig (Porcine), Mouse (Murine), Plant
(245), (128), (127), (32), (25), (17), (14), (13), (13), (11), (10), (9), (8), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(159), (137), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB antibody is un-conjugated
(10), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(247), (91), (89), (76), (58), (46), (23), (18), (9), (7), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB Antibody (ABIN192340) Images
Specificity The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen beta-tubulin from porcine brain
Clone TU-13
Isotype IgM

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB Antibody (ABIN192340) Images back to top
Antigen
Alternative Name TUBB (TUBB Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening -, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB Antibody (ABIN192340) Images back to top
Application Notes Western Blotting: Recommended dilution: 1 µg/mL
Positive control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa human cell line, EL4 mouse cell line, MCF7 human cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Application note: Reducing conditions. Sonication of MCF7 cells is recommended.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB Antibody (ABIN192340) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-TUBB Antibody (ABIN192340)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Background publications

Linhartová, Dráberová, Viklický, Dráber: "Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells." in: FEBS letters, Vol. 320, Issue 1, pp. 79-82, 1993

Product cited in:

Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, 1990

Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, 1988