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anti-All Species TUBB3 antibody for Western Blotting

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin, beta 3 class III
  • beta-tubulin 3
  • similar to tubulin, beta 3
  • tubulin, beta, 4
  • tubulin, beta 3
  • beta tubulin3
  • tubulin, beta 2C
  • tubulin, beta 4A class IVA
  • tubb3
  • TUBB3
  • tub3
  • TUBB2C
  • Tubb3
  • Tubb4a
Epitope
AA 441-448, C-Term, N-Term
44
43
20
15
12
6
5
4
4
4
4
3
3
2
2
1
1
1
1
Reactivity
All Species, Human, Mouse (Murine)
233
110
98
21
19
15
15
8
8
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
128
121
16
14
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is conjugated to FITC
25
14
13
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
235
104
85
64
63
52
46
17
13
13
12
8
6
6
2
2
2
2
2
Supplier
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Catalog No. ABIN269248
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN153066 ICC FACS IF IHC (p) IHC WB Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20 9
1 ABIN93912 FACS ICC IHC (p) WB FITC Mouse IgG1 N-Term, C-Term, AA 441-448 Log in to see TU-20 10
1 ABIN93911 ICC FACS IHC (p) WB Mouse IgG1 N-Term Log in to see TU-20 14
1 ABIN4284549 FACS ICC IF IHC IHC (p) WB PerCP Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284558 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284547 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284546 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284551 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284552 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284553 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284556 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284559 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284557 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN3199342 FACS ICC IHC (p) WB FITC Mouse IgG1 N-Term Log in to see TU-20
1 ABIN3200832 FACS ICC IF IHC (p) WB Mouse IgG1 AA 441-448, C-Term Log in to see TU-20
1 ABIN3036355 FACS IF/ICC IHC WB Mouse IgG1 N-Term Log in to see
1 ABIN3036356 FACS IF/ICC IHC WB FITC Mouse IgG1 N-Term Log in to see

Top referenced anti-TUBB3 antibody for Western Blotting

Similar anti-TUBB3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 20 Antibodies 85 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunohistochemistry (Whole Mount) (IHC (wm)) 2 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 87 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 441-448, C-Term, N-Term
(44), (43), (20), (15), (12), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity All Species, Human, Mouse (Murine)
(233), (110), (98), (21), (19), (15), (15), (8), (8), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(128), (121), (16), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is conjugated to FITC
(25), (14), (13), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(235), (104), (85), (64), (63), (52), (46), (17), (13), (13), (12), (8), (6), (6), (2), (2), (2), (2), (2)
Pubmed 10 references available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) Images
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Purification Protein A or G purified
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) Images back to top
Antigen
Alternative Name TUBB3 / TUBB4 (TUBB3 Antibody Abstract)
Background Gene Symbol: TUBB3
Gene ID 10381
Pathways Microtubule Dynamics, M Phase

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN269248) Images back to top
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:40, Immunohistochemistry-Paraffin 10 μg/mLFor Immunohistochemistry-Paraffin: Use standard ABC technique (DAB+). Purity is > 95 % (by SDS-PAGE). Western Blot: reducing conditions
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN269248) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.

References for anti-TUBB3 Antibody (ABIN269248)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (FITC) (ABIN269248) anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (FITC)