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anti-General TUBB3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Epitope
N-Term
33
27
11
7
5
5
5
4
4
4
3
3
2
2
1
Reactivity
General
189
55
52
11
4
4
4
2
2
1
1
1
Host
Mouse
101
89
14
12
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is un-conjugated
23
12
11
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
177
80
80
43
41
35
33
16
13
9
6
2
2
2
Supplier
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Catalog No. ABIN93911
$ 277.20
Plus shipping costs $45.00

Top referenced anti-TUBB3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-TUBB3 Antibodies

Application / Reactivity General
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 1 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope N-Term
(33), (27), (11), (7), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1)
Reactivity General
(189), (55), (52), (11), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(101), (89), (14), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is un-conjugated
(23), (12), (11), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(177), (80), (80), (43), (41), (35), (33), (16), (13), (9), (6), (2), (2), (2)
Pubmed 14 references available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN93911) Images
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Purification Purified by ammonium sulphate and caprylic acid precipitation
Purity > 95 % (by SDS-PAGE)
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN93911) Images back to top
Antigen
Alternative Name betaIII-tubulin (TUBB3 Antibody Abstract)
Background The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN93911) Images back to top
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl,
Incubation 30 min in RT, or High temperature citrate buffer antigen retrieval
Positive tissue: neuronal tissueImmunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN93911) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-TUBB3 Antibody (ABIN93911)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011

Kunova, Matulka, Eiselleova, Trckova, Hampl, Dvorak: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998

Background publications

Silva, Pereira, Oliveira, Relvas, Rego: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009

Woltjen, Michael, Mohseni, Desai, Mileikovsky, Haemaelaeinen, Cowling, Wang, Liu, Gertsenstein, Kaji, Sung, Nagy: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN93911) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining o...
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to...
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence stai...