You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CD40 Ligand antibody for Western Blotting

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
  • LOC100358388
  • CD154
Epitope
C-Term
21
16
15
15
14
13
9
8
7
7
6
6
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
454
234
55
20
20
10
8
8
5
5
4
3
3
3
3
2
2
1
Host
Rabbit
276
166
106
51
35
26
1
Clonality
Polyclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
73
58
43
14
14
12
12
11
9
8
8
8
8
8
8
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
400
270
170
93
66
51
49
42
33
20
16
13
11
9
7
7
5
5
4
4
3
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN453857
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0411053 ABIN453437 EIA WB Rabbit N-Term Log in to see Polyclonal 2
3.0411053 ABIN4899210 FACS WB Goat IgG AA 108-261 Log in to see Polyclonal 2
3.0411053 ABIN3044319 IHC (p) WB Rabbit IgG AA 47-65, N-Term Log in to see Polyclonal
3.0411053 ABIN390458 FACS IF IHC (p) WB Rabbit Ig AA 33-62, N-Term Log in to see Polyclonal
3.0411053 ABIN2705743 WB Rabbit Center Log in to see Polyclonal
3.0411053 ABIN1105902 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.0411053 ABIN652211 FACS IHC (p) WB Rabbit Ig AA 181-207, C-Term Log in to see Polyclonal
3.0411053 ABIN222966 WB Rabbit IgG Log in to see Polyclonal
3.0411053 ABIN394480 WB Mouse IgG2b kappa AA 101-204 Log in to see 2E2
3.0411053 ABIN2854749 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.0411053 ABIN1871635 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0411053 ABIN223518 WB Rabbit IgG Log in to see Polyclonal
3.0411053 ABIN3020937 IHC WB Rabbit IgG N-Term Log in to see
3.0411053 ABIN1858302 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 180-261 Log in to see
3.0411053 ABIN1858301 ICC IHC IP WB Rabbit IgG AA 48-244 Log in to see Polyclonal
3.0411053 ABIN1172349 ICC IHC IP WB Rabbit IgG AA 180-261 Log in to see Polyclonal
3.0411053 ABIN3028551 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 33-62 Log in to see Polyclonal
3.0411053 ABIN4292978 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.0411053 ABIN560252 ELISA WB Mouse IgG2b kappa AA 101-203, partial Log in to see 2E2
3.0411053 ABIN1980386 ICC IHC IP WB Rabbit IgG AA 1-261 Log in to see Polyclonal

Top referenced anti-CD40 Ligand antibody for Western Blotting

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
ELISA 149 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 231 Antibodies
Functional Studies (Func) 26 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 50 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope C-Term
(21), (16), (15), (15), (14), (13), (9), (8), (7), (7), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(454), (234), (55), (20), (20), (10), (8), (8), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1)
Host Rabbit
(276), (166), (106), (51), (35), (26), (1)
Clonality
Polyclonal
Conjugate This CD40 Ligand antibody is un-conjugated
(73), (58), (43), (14), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(400), (270), (170), (93), (66), (51), (49), (42), (33), (20), (16), (13), (11), (9), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453857) Images
Specificity This antibody reacts to CD154.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the C-terminal region of human TRAP

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453857) Images back to top
Antigen
Alternative Name CD154 / CD40L (CD40LG Antibody Abstract)
Background TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
Gene ID 959
NCBI Accession NP_000065
UniProt P29965
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN453857) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN453857) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Ligand Antibody (ABIN453857)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Background publications

Volmar, Ait-Ghezala, Frieling, Weeks, Mullan: "CD40/CD40L interaction induces Abeta production and increases gamma-secretase activity independently of tumor necrosis factor receptor associated factor (TRAF) signaling." in: Experimental cell research, Vol. 315, Issue 13, pp. 2265-74, 2009

Chai, Aghaie, Zhou: "Soluble CD40 ligand induces human coronary artery smooth muscle cells proliferation and migration." in: Surgery, Vol. 146, Issue 1, pp. 5-11, 2009

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453857) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 Ligand antibody (CD40LG) (C-Term) (ABIN453857) Western blot analysis of TRAP Antibody (C-term) in K562 cell line lysates (35ug/lane)...