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anti-Human CD40 Ligand antibody for Western Blotting

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Epitope
N-Term
20
15
14
12
8
6
6
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
365
152
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Rabbit
219
122
89
26
21
19
Clonality
Polyclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
72
54
36
8
6
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
271
189
135
53
51
49
40
31
22
15
11
7
6
6
5
5
4
3
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN453437
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12316538 ABIN453857 EIA FACS WB Rabbit C-Term Log in to see Polyclonal 2
0.12316538 ABIN390458 FACS IF IHC (p) WB Rabbit Ig AA 33-62, N-Term Log in to see Polyclonal
0.12316538 ABIN222966 WB Rabbit IgG Log in to see Polyclonal
0.12316538 ABIN1105902 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.12316538 ABIN2705743 WB Rabbit Center Log in to see Polyclonal
0.12316538 ABIN652211 FACS IHC (p) WB Rabbit Ig AA 181-207, C-Term Log in to see Polyclonal
0.12316538 ABIN394480 WB Mouse IgG2b kappa AA 101-204 Log in to see 2E2
0.12316538 ABIN258522 WB Rabbit IgG Log in to see EP462E
0.12316538 ABIN223518 WB Rabbit IgG Log in to see Polyclonal
0.12316538 ABIN3044319 IHC (p) WB Rabbit IgG AA 47-65, N-Term Log in to see Polyclonal
0.12316538 ABIN1871635 IHC WB Rabbit IgG Log in to see Polyclonal
0.12316538 ABIN2854749 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.12316538 ABIN3020937 IHC WB Rabbit IgG N-Term Log in to see
0.12316538 ABIN1858302 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 180-261 Log in to see
0.12316538 ABIN1858301 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 48-244 Log in to see Polyclonal
0.12316538 ABIN634665 WB Rabbit Log in to see Polyclonal
0.12316538 ABIN2166700 WB FITC Rat IgG2a Log in to see
0.12316538 ABIN2166701 WB FITC Rat IgG2a Log in to see
0.12316538 ABIN560252 ELISA WB Mouse IgG2b kappa AA 101-203, partial Log in to see 2E2
0.12316538 ABIN1172349 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 180-261 Log in to see Polyclonal

Top referenced anti-CD40 Ligand antibody for Western Blotting

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 121 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 175 Antibodies
Functional Studies (Func) 30 Antibodies
Immunoassay (IA) 7 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 39 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 9 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope N-Term
(20), (15), (14), (12), (8), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(365), (152), (40), (15), (14), (11), (7), (4), (3), (3), (3), (2), (2), (2), (2)
Host Rabbit
(219), (122), (89), (26), (21), (19)
Clonality
Polyclonal
Conjugate This CD40 Ligand antibody is un-conjugated
(72), (54), (36), (8), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(271), (189), (135), (53), (51), (49), (40), (31), (22), (15), (11), (7), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453437) Images
Specificity This antibody reacts to CD154 / CD40L.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human TRAP

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453437) Images back to top
Antigen
Alternative Name CD154 / CD40L (CD40LG Antibody Abstract)
Background TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
Gene ID 959
NCBI Accession NP_000065
UniProt P29965
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN453437) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN453437) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Ligand Antibody (ABIN453437)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Background publications

Chai, Aghaie, Zhou: "Soluble CD40 ligand induces human coronary artery smooth muscle cells proliferation and migration." in: Surgery, Vol. 146, Issue 1, pp. 5-11, 2009

Volmar, Ait-Ghezala, Frieling, Weeks, Mullan: "CD40/CD40L interaction induces Abeta production and increases gamma-secretase activity independently of tumor necrosis factor receptor associated factor (TRAF) signaling." in: Experimental cell research, Vol. 315, Issue 13, pp. 2265-74, 2009

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN453437) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 Ligand antibody (CD40LG) (N-Term) (ABIN453437) Western blot analysis of TRAP antibody (N-term) in NCI-H460 cell line lysates (35ug/l...