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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Confocal Microscopy (CM)||4 Antibodies||4 Antibodies|
|Dot Blot (DB)||2 Antibodies||2 Antibodies|
|ELISA||13 Antibodies||11 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||6 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||6 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Neutralization (Neut)||5 Antibodies|
|Western Blotting (WB)||25 Antibodies|
|Antigen||Growth Hormone 1 (GH1) Antibodies|
|Epitope||AA 172-192, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Growth Hormone 1 antibody is un-conjugated Alternatives|
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
|2 references available|
|Supplier||Log in to see|
Product Details anti-Growth Hormone 1 AntibodyTarget Details Growth Hormone 1 Application Details Handling References for anti-Growth Hormone 1 Antibody (ABIN493010) Images
|Sequence||DKV ETF LRI VQC RSV EGS CGF|
|Specificity||This antibody labels gonadotrophs in pituitary sections. The antibody does not react to other cellular proteins that are tested during characterization of this antibody.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Immunogen||Synthetic peptide corresponding to amino acids 172-192|
Target Details Growth Hormone 1Product Details anti-Growth Hormone 1 Antibody Application Details Handling References for anti-Growth Hormone 1 Antibody (ABIN493010) Images back to top
|Alternative Name||Somatotropin / Growth Hormone / GH1 (GH1 Antibody Abstract)|
|Background||The peptide hormones of the anterior pituitary are essential for growth, reproduction and intermediary metabolism. Their synthesis and secretion is controlled by hormones of hypothalamic origin, diseases and several pharmacological agents. In vertebrates 10 anterior pituitary hormones have been identified and are classified in to three classes based on their structural features. The growth hormone (GH), prolactin (Prl) and placental lactogen (PL) are members of somototropic hormone family. The glycoprotein hormone family comprise of Thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH). The corticotrophin (ACTH), two melanocyte stimulating hormones (MSH) and the two lipotropins (LPH) are the members of hormones derived from POMC gene. The human growth hormone (hGH) is coded by one gene of a five gene cluster located on chromosome 17, the other genes code for two other variants of lactogen or a growth hormone variant expressed in placenta. The hGH is a single polypeptide chain with 191 amino acids (22 kDa). The hGH has 2 disulfide bonds and it is not glycosylated. There exist a splice variant of hGH where amino acids 32-46 is not translated and this splice variant accounts up to 5-10 % of the total hGH. Placenta also secrete a 22 kDa hGH that can be distinguished from the pituitary form but its significance is not clear. The amount of GH secreted in 24 hours is high in children reaches maximal levels at the time of adolescence then tapers off during adult hood. The secretion of GH is in pulses and occurs at irregular intervals. The amplitude of GH concentration is maximum at night shortly after onset of REM sleep. Somatotrophs in anterior pituitary have an intracellular Ca+2 oscillations that are believed to to be responsible for pulsatile secretion of GH (1). The release of GH is under direct control of GH release hormone (GHRH) which stimulates GH. Both GH and GHRH bind to their cognate GPCR leading to an increase (GHRH) or decrease (somatotropin) in cAMP and calcium levels. Several pharmacological agents can increase GH release via acting on hypothalamus. The physiological effects of GH is via its interaction with cell surface receptor which are widely distributed throughout body. The GH receptor is a 620 amino acid single trans-membrane protein with large extracellular N-terminal domain that interacts with GH and a about 250 amino acid cytoplasmic end of the protein. The large extracellular domain of GH receptor is cleaved to acts as GH binding protein of about 60 kDa. The GH has direct effect on lipid and carbohydrate metabolism, its anabolic growth promoting effects are mediated by somatomedins or insulin like growth factors (IGF-1 and IGF-2) which share significant homology (2). GH administration increase the production of IGF in liver, triglyceride hydrolysis and hepatic glucose output. IGF-1 also exerts indirect growth promoting effects of GH by increase chondrogenesis, skeletal and soft tissue growth.Synonyms: Growth hormone 1, HGH, Pituitary growth hormone|
|Pathways||NF-kappaB Signaling, JAK-STAT Signaling|
Application DetailsProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Handling References for anti-Growth Hormone 1 Antibody (ABIN493010) Images back to top
Immunohistochemistry on frozen sections: 1: 250. Dot blot. ELISA: 1: 50,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details References for anti-Growth Hormone 1 Antibody (ABIN493010) Images back to top
|Buffer||Stabilization buffer with 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-Growth Hormone 1 Antibody (ABIN493010)Product Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Handling Images back to top
Sun, Zhou, Fang et al.: "Hollow sinh-Gaussian beams and their paraxial properties." in: Optics express, Vol. 20, Issue 9, pp. 9682-91, 2012 (PubMed).
Holl, Thorner, Mandell et al.: "Spontaneous oscillations of intracellular calcium and growth hormone secretion." in: The Journal of biological chemistry, Vol. 263, Issue 20, pp. 9682-5, 1988 (PubMed).