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|Application / Reactivity||Rat (Rattus)|
|Confocal Microscopy (CM)||4 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Neutralization (Neut)||5 Antibodies|
|Western Blotting (WB)||25 Antibodies|
|Antigen||Growth Hormone 1 (GH1) Antibodies|
|Epitope||AA 27-216 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Growth Hormone 1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-Growth Hormone 1 AntibodyTarget Details Growth Hormone 1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Rat.
Gene Name: growth hormone 1
Protein Name: Somatotropin
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived rat GH recombinant protein(Position: F27-F216).|
Target Details Growth Hormone 1Product Details anti-Growth Hormone 1 Antibody Application Details Handling Images back to top
|Alternative Name||GH1 (GH1 Antibody Abstract)|
|Background||Growth Hormone(GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98 % amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.|
|Pathways||NF-kappaB Signaling, JAK-STAT Signaling|
Application DetailsProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Rat
Neut: Species: Rat
IP: Species: Rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Handling back to top