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anti-Mouse (Murine) IKBKB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • ikk-beta
  • ikk2
  • ikkb
  • ikkbeta
  • IKBKB
  • IKKB
  • CG4201
  • DIK
  • DLAK
  • DmIKK
  • DmIKKBeta
  • DmIKKb
  • DmIKKbeta
  • DmIkkbeta
  • Dmel\\CG4201
  • Dmikkb
  • Dmikkbeta
  • IK
  • IKK
  • IKK-beta
  • IKKb
  • IKKbeta
  • IRD-5
  • IRD5
  • Ik
  • Ikkb
  • Ikkbeta
  • Ird-5
  • Ird5
  • LRK
  • Lak
  • anon-89Bd
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • dLak
  • dmIKKbeta
  • ikk
  • ird
  • ird5/DmIkk[beta]
  • IKK2
  • NFKBIKB
  • AIM-1
  • AI132552
  • IKK-2
  • IKK[b]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • immune response deficient 5
  • inhibitor of kappaB kinase beta
  • ikbkb
  • IKBKB
  • ird5
  • Ikbkb
Reactivity
Human, Mouse (Murine)
655
227
159
22
15
7
5
5
4
2
1
1
1
Host
Mouse
554
115
9
2
Clonality
Monoclonal
Conjugate
This IKBKB antibody is un-conjugated
38
37
37
33
31
31
9
7
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
311
277
264
99
74
72
68
40
36
33
6
6
6
4
3
3
2
1
1
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Catalog No. ABIN252473
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.701424 ABIN269739 IHC (p) IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
11.701424 ABIN743243 IF (p) IHC (p) WB Rabbit IgG AA 180-230, pTyr199 Log in to see Polyclonal
11.701424 ABIN408033 IHC (p) IHC Rabbit IgG Log in to see Polyclonal
11.701424 ABIN297183 IHC (p) WB Chicken IgY AA 310-376 Log in to see Polyclonal
11.701424 ABIN218607 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN271895 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN272025 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN400933 IHC (p) WB Chicken Ig AA 310-376 Log in to see Polyclonal
1 ABIN4324294 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see 10AG2
1 ABIN4324261 IHC IHC (p) WB Rabbit Log in to see Polyclonal 2
1 ABIN2878951 IHC (p) ELISA Rabbit IgG AA 166-215 Log in to see Polyclonal
1 ABIN1735855 IHC (p) ELISA Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN743252 IHC (p) WB HRP Rabbit IgG AA 180-230, pTyr199 Log in to see Polyclonal
1 ABIN743245 IHC (p) WB Biotin Rabbit IgG AA 180-230, pTyr199 Log in to see Polyclonal
1 ABIN4324272 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 Log in to see 10AG2
1 ABIN4324295 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see 10AG2
1 ABIN4324293 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see 10AG2
1 ABIN4324277 FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 Log in to see 10AG2
1 ABIN4324264 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 Log in to see 10AG2
1 ABIN4324270 FACS ICC IF IHC IHC (p) WB DyLight 650 Mouse IgG1 Log in to see 10AG2

Top referenced anti-IKBKB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-IKBKB Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
Dot Blot (DB) 21 Antibodies 243 Antibodies
ELISA 43 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 36 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Simple Western (SimWes) 5 Antibodies
Western Blotting (WB) 123 Antibodies

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Reactivity Human, Mouse (Murine)
(655), (227), (159), (22), (15), (7), (5), (5), (4), (2), (1), (1), (1)
Host Mouse
(554), (115), (9), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IKBKB antibody is un-conjugated
(38), (37), (37), (33), (31), (31), (9), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(311), (277), (264), (99), (74), (72), (68), (40), (36), (33), (6), (6), (6), (4), (3), (3), (2), (1), (1)
Pubmed 20 references available
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN252473) Images
Specificity An approx. 87 kDa band should be observed.
Purification Protein G purified
Immunogen A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Clone 10AG2
Isotype IgG1

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN252473) Images back to top
Antigen
Alternative Name IKK beta (IKBKB Antibody Abstract)
Background Gene Symbol: IKBKB
Molecular Weight Theoretical MW: 86.564 kDa
Gene ID 3551
UniProt O14920
Research Area Immunology, Innate Immunity, Atherosclerosis, Transcription Factors, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN252473) Images back to top
Application Notes Western Blot 2 - 4 μg/mL, Simple Western 1:12.5, Flow Cytometry 0.25 - 0.5 μg/ 10^6 cells, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, CyTOF-readyUseful in Immunohistochemistry-Paraffin See Page et al. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24825920) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN252473) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-IKBKB Antibody (ABIN252473)

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Product cited in:

Liu, Ibi, Taniguchi, Lee, Herrema, Akosman, Mucka, Salazar Hernandez, Uyar, Park, Karin, Ozcan: "Inflammation Improves Glucose Homeostasis through IKKβ-XBP1s Interaction." in: Cell, Vol. 167, Issue 4, pp. 1052-1066.e18, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (Sample species: Human). Further details: Western Blotting

Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Breuer, Becker, Brechmann, Mock, Arnold, Krammer: "The protein phosphatase 2A regulatory subunit B56γ mediates suppression of T cell receptor (TCR)-induced nuclear factor-κB (NF-κB) activity." in: The Journal of biological chemistry, Vol. 289, Issue 21, pp. 14996-5004, 2014 (Sample species: Human). Further details: Western Blotting

Paul, Traver, Kashyap, Washington, Latoche, Schaefer: "T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome." in: Science signaling, Vol. 7, Issue 325, pp. ra45, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (Sample species: Human). Further details: Western Blotting

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011 (Sample species: Human). Further details: Western Blotting

Page, Cascallana, Casanova, Navarro, Alameda, Pérez, Bravo, Ramírez: "IKKβ overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia." in: Molecular cancer research : MCR, Vol. 9, Issue 10, pp. 1329-38, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting

Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010

Huang, Ren, Guan, Jiang, Cheng: "HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6208-17, 2009 (Sample species: Human). Further details: Western Blotting

Koch, Maeda, He, Karin, Leffert: "Targeted deletion of hepatocyte Ikkbeta confers growth advantages." in: Biochemical and biophysical research communications, Vol. 380, Issue 2, pp. 349-54, 2009 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Jin, Lee, Kim, Chung, Lee, Lee: "cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation." in: Cancer research, Vol. 69, Issue 5, pp. 1782-91, 2009 Further details: Immunoprecipitation

Kasinski, Du, Thomas, Zhao, Sun, Khuri, Wang, Shoji, Sun, Snyder, Liotta, Fu: "Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin." in: Molecular pharmacology, Vol. 74, Issue 3, pp. 654-61, 2008 (Sample species: Human). Further details: Western Blotting

Pandey, Sung, Kunnumakkara, Sethi, Chaturvedi, Aggarwal: "Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis." in: Cancer research, Vol. 68, Issue 13, pp. 5370-9, 2008 (Sample species: Human). Further details: Western Blotting

Kunnumakkara, Ichikawa, Anand, Mohankumar, Hema, Nair, Aggarwal et al.: "Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of ..." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3306-17, 2008 (Sample species: Human). Further details: Western Blotting

Leis, Sanchis, Pérez: "Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway." in: Experimental cell research, Vol. 313, Issue 4, pp. 742-52, 2007 (Sample species: Mouse (Murine)).

Jackson-Bernitsas, Ichikawa, Takada, Myers, Lin, Darnay, Chaturvedi, Aggarwal: "Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma." in: Oncogene, Vol. 26, Issue 10, pp. 1385-97, 2007 Further details: Western Blotting

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN252473) back to top
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mo...
Simple Western (SimWes) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Simple Western: IKK beta Antibody (10AG2) [ABIN2524739] - Simple Western lane view sh...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis using the Alexa Fl...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis of PE conjugate of...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis using Alexa Fluor ...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Intracellular staining of H...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [ABIN2524739] - Formalin-fix...