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anti-Mouse (Murine) IKBKB antibody for Western Blotting

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • ikk-beta
  • ikk2
  • ikkb
  • ikkbeta
  • IKBKB
  • IKKB
  • CG4201
  • DIK
  • DLAK
  • DmIKK
  • DmIKKBeta
  • DmIKKb
  • DmIKKbeta
  • DmIkkbeta
  • Dmel\\CG4201
  • Dmikkb
  • Dmikkbeta
  • IK
  • IKK
  • IKK-beta
  • IKKb
  • IKKbeta
  • IRD-5
  • IRD5
  • Ik
  • Ikkb
  • Ikkbeta
  • Ird-5
  • Ird5
  • LRK
  • Lak
  • anon-89Bd
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • dLak
  • dmIKKbeta
  • ikk
  • ird
  • ird5/DmIkk[beta]
  • IKK2
  • NFKBIKB
  • AIM-1
  • AI132552
  • IKK-2
  • IKK[b]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • immune response deficient 5
  • inhibitor of kappaB kinase beta
  • ikbkb
  • IKBKB
  • ird5
  • Ikbkb
Epitope
Middle Region
51
32
28
22
22
22
21
21
21
21
20
20
20
20
20
20
20
16
16
16
15
8
8
8
8
7
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
679
234
163
21
14
7
5
4
4
2
1
1
1
Host
Rabbit
577
117
9
2
Clonality
Polyclonal
Conjugate
This IKBKB antibody is un-conjugated
38
37
37
33
31
31
9
7
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
318
288
278
106
74
74
68
43
37
34
6
6
6
5
3
3
2
2
1
1
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Catalog No. ABIN2779400
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1003003 ABIN2779787 WB Rabbit N-Term Log in to see Polyclonal 1
3.1003003 ABIN252473 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 Log in to see 10AG2 56
3.1003003 ABIN252477 FACS GS ICC IF IP SimWes WB Mouse IgG1 kappa Log in to see 10A9B6 9
3.1003003 ABIN269739 IHC (p) IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
3.1003003 ABIN1531286 IHC ELISA WB Rabbit IgG pTyr188 Log in to see Polyclonal
3.1003003 ABIN1531325 IHC ELISA WB Rabbit IgG pTyr199 Log in to see Polyclonal
3.1003003 ABIN3185150 ELISA IHC WB Rabbit IgG Ser532 Log in to see Polyclonal
3.1003003 ABIN2706368 IP IHC WB Rabbit pTyr199 Log in to see Polyclonal
3.1003003 ABIN2707593 WB Rabbit N-Term Log in to see Polyclonal
3.1003003 ABIN2707591 IC IF IHC WB Rabbit N-Term, pSer23 Log in to see Polyclonal
3.1003003 ABIN2707592 IHC WB Rabbit N-Term, pSer19 Log in to see Polyclonal
3.1003003 ABIN2706367 IHC WB Rabbit pTyr188 Log in to see Polyclonal
3.1003003 ABIN4324261 IHC IHC (p) WB Rabbit Log in to see Polyclonal 2
3.1003003 ABIN1532306 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN1539739 DB WB Rabbit Ig pTyr1140 Log in to see Polyclonal
3.1003003 ABIN1873178 WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN4324301 FACS GS ICC IF IP SimWes WB Mouse IgG1 kappa Log in to see 10A9B6
3.1003003 ABIN2490844 IHC ELISA WB Rabbit IgG pSer176, pSer177 Log in to see Polyclonal
3.1003003 ABIN2490843 IHC ELISA WB Rabbit IgG Tyr188 Log in to see Polyclonal
3.1003003 ABIN2490845 IHC ELISA WB Rabbit IgG pTyr188 Log in to see Polyclonal

Top referenced anti-IKBKB antibody for Western Blotting

Similar anti-IKBKB Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Pig (Porcine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 4 Antibodies 3 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies
Western Blotting (WB) 285 Antibodies 128 Antibodies 87 Antibodies 16 Antibodies 3 Antibodies 11 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies
Immunoprecipitation (IP) 33 Antibodies 21 Antibodies 10 Antibodies 3 Antibodies 3 Antibodies

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Epitope Middle Region
(51), (32), (28), (22), (22), (22), (21), (21), (21), (21), (20), (20), (20), (20), (20), (20), (20), (16), (16), (16), (15), (8), (8), (8), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(679), (234), (163), (21), (14), (7), (5), (4), (4), (2), (1), (1), (1)
Host Rabbit
(577), (117), (9), (2)
Clonality
Polyclonal
Conjugate This IKBKB antibody is un-conjugated
(38), (37), (37), (33), (31), (31), (9), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(318), (288), (278), (106), (74), (74), (68), (43), (37), (34), (6), (6), (6), (5), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN2779400) Images
Blocking Peptide Blocking peptide for this product available: ABIN978456
Sequence ITYETQISPR PQPESVSCIL QEPKRNLAFF QLRKVWGQVW HSIQTLKEDC
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 90 %, Pig : 90 %, Rat : 90 %, Bovine : 70 %
Characteristics This is a rabbit polyclonal antibody against IKBKB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IKBKB

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN2779400) Images back to top
Antigen
Alternative Name IKBKB (IKBKB Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Molecular Weight 86 kDa
Gene ID 3551
NCBI Accession NM_001556, NP_001547
UniProt O14920
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN2779400) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 756 AA

Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN2779400) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IKBKB Antibody (ABIN2779400)

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Background publications

Hu, Li, Mahavadi, Murthy: "Interleukin-1beta up-regulates RGS4 through the canonical IKK2/IkappaBalpha/NF-kappaB pathway in rabbit colonic smooth muscle." in: The Biochemical journal, Vol. 412, Issue 1, pp. 35-43, 2008

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN2779400) back to top
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (Middle Region) (ABIN2779400) WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: