Human IKBKG ELISA Kit

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Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) ELISA Kits
  • nemo
  • fj33c07
  • wu:fj33c07
  • zgc:113492
  • MGC97885
  • IKBKG
  • IKBKG_1
  • IKBKG_2
  • CG16910
  • DmIKK-gamma
  • DmIKKgamma
  • Dmel\\CG16910
  • Dmikkgamma
  • IKK
  • IKK-gamma
  • IKK[[gamma]]
  • IKKg
  • IKKgamma
  • KEY
  • Kenny
  • Key
  • dIKK
  • dIKK-gamma
  • dmIKKgamma
  • AMCBX1
  • FIP-3
  • FIP3
  • Fip3p
  • IP
  • IP1
  • IP2
  • IPD2
  • NEMO
  • ZC2HC9
  • Nemo
  • 1110037D23Rik
  • AI848108
  • AI851264
  • AW124339
  • IKK[g]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • kenny
  • inhibitor of kappaB kinase gamma
  • ikbkg
  • IKBKG
  • LOC782205
  • IKBKG_1
  • key
  • Ikbkg
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
12
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.15-10 ng/mL
Minimum Detection Limit
0.15 ng/mL
Application
ELISA
Options
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Catalog No. ABIN5564696
$ 589.47
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN5593823 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN1380483 Cell ELISA Log in to see
1 ABIN1380184 Cell ELISA Log in to see
1 ABIN5523402 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN1381230 Cell ELISA Log in to see
1 ABIN2114626 Cell ELISA Log in to see
1 ABIN2114625 Cell ELISA Log in to see
1 ABIN2114624 Cell ELISA Log in to see
1 ABIN1145038 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN2684552 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2949913 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN5071273 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(12), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.15-10 ng/mL
Minimum Detection Limit 0.15 ng/mL
Application ELISA
Supplier Log in to see

Product Details IKBKG ELISA Kit

Target details Application Details Handling Images
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg).
No significant cross-reactivity or interference between Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) and analogues was observed.

Sensitivity 0.064 ng/mL
Material not included
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

Product Details IKBKG ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) (IKBKG ELISA Kit Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details IKBKG ELISA Kit Target details Handling Images back to top
Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure
  1. Prepare all reagents, samples and standards,
  2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
  3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
  4. Aspirate and wash 3 times,
  5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  6. Aspirate and wash 5 times,
  7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

Product Details IKBKG ELISA Kit Target details Application Details Images back to top
Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C

Images

Product Details IKBKG ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) ELISA Kit (ABIN5564696) Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) ELISA Kit