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anti-Mouse (Murine) IL1A antibody for ELISA (Capture)

Recommended IL1A Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 alpha (IL1A) Antibodies
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Reactivity
Mouse (Murine)
248
102
53
8
7
7
7
6
5
3
2
1
1
Host
Armenian Hamster
222
128
34
10
7
7
6
3
3
Clonality
Monoclonal
Conjugate
This IL1A antibody is un-conjugated
68
31
15
14
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture)
276
271
61
56
51
33
21
20
20
18
16
10
9
5
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN281599
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Top referenced anti-IL1A antibody for ELISA (Capture)

Similar anti-IL1A Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
ELISA 64 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 10 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 53 Antibodies

General

Antigen Interleukin 1 alpha (IL1A) Antibodies
Reactivity Mouse (Murine)
(248), (102), (53), (8), (7), (7), (7), (6), (5), (3), (2), (1), (1)
Host Armenian Hamster
(222), (128), (34), (10), (7), (7), (6), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1A antibody is un-conjugated
(68), (31), (15), (14), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture)
(276), (271), (61), (56), (51), (33), (21), (20), (20), (18), (16), (10), (9), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1A Antibody

Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN281599) Images
Specificity The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23 % amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Characteristics The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23 % amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Synonyms: Synonyms: Interleukin-1 alpha, IL-1a, IL1a
Purification Affinity Purified
Clone ALF-161
Isotype IgG

Target Details IL1A

Product Details anti-IL1A Antibody Application Details Handling References for anti-IL1A Antibody (ABIN281599) Images back to top
Antigen
Alternative Name IL-1a (IL1A Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details anti-IL1A Antibody Target Details IL1A Handling References for anti-IL1A Antibody (ABIN281599) Images back to top
Application Notes Applications reported: The ALF-161 antibody has been reported for use in ELISA capture.

Applications tested: The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1 alpha) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1a (14- 8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL1A Antibody Target Details IL1A Application Details References for anti-IL1A Antibody (ABIN281599) Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

References for anti-IL1A Antibody (ABIN281599)

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling Images back to top
Background publications

Rogers, Sheehan, Brunt, Dower, Unanue, Schreiber: "Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 3, pp. 1011-5, 1992

Images

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN281599) back to top
Supplier Images
 image for anti-IL1A antibody (Interleukin 1 alpha) (ABIN281599) Mouse splenocytes (500,000 cells per well) were activated with LPS (1 µg/ml) for 48 h...
 image for anti-IL1A antibody (Interleukin 1 alpha) (ABIN281599) anti-Interleukin 1 alpha (IL1A) antibody (Image 2)