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|Application / Reactivity||Mouse (Murine)|
|Activation (Act)||1 Antibodies|
|Blocking Antibody (Inhibition)||3 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||16 Antibodies|
|Functional Studies (Func)||10 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Neutralization (Neut)||16 Antibodies|
|Western Blotting (WB)||53 Antibodies|
|Antigen||Interleukin 1 alpha (IL1A) Antibodies|
|Epitope||AA 256-270, C-Term Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL1A antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IL1A AntibodyTarget Details IL1A Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Interleukin-1 alpha(IL1A) detection. Tested with WB, IHC-P in Mouse.
Gene Name: interleukin 1, alpha
Protein Name: Interleukin-1 alpha(IL-1 alpha)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL1 alpha(256-270aa HLARGLPSMTDFQIS).|
Target Details IL1AProduct Details anti-IL1A Antibody Application Details Handling Images back to top
|Alternative Name||IL1A (IL1A Antibody Abstract)|
|Background||Interleukin-1 alpha(IL-1alpha) is a protein that in humans is encoded by the IL1A gene. The protein encoded by this gene is a cytokine of the interleukin-1 family. Interleukin-1 alpha possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. Silver et al.(1990) showed that the mouse Il1a and Il1b genes are contained in a genomic fragment of about 70 kb. Further studies suggested that Il1b lies 5-prime to Il1a, that the 2 genes are oriented in the same direction, and that they are separated by about 50 kb.|
|Pathways||NF-kappaB Signaling, TCR Signaling|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1A Antibody Target Details IL1A Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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