anti-Human Insulin antibody for Enzyme Immunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Human
949
397
284
274
262
121
49
6
6
5
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
835
182
54
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
56
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
622
371
247
233
204
204
161
138
70
66
40
27
16
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN111474
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN112214 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 1
1 ABIN111464 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see C-PEP-01 2
1 ABIN111475 EIA IHC (fro) IHC (p) RIA Mouse IgG1 Log in to see C-PEP-01 1
1 ABIN372838 EIA FACS IF IHC (p) Rabbit IgG Log in to see Polyclonal 3
1 ABIN5556681 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 2D11-H5
1 ABIN111476 EIA RIA Mouse IgG1 Log in to see C-PEP-01 1
1 ABIN111965 EIA Func IHC (p) RIA Mouse IgG1 Log in to see IN-05 1
1 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
1 ABIN316680 EIA IHC (p) Mouse IgG1 Log in to see 2D11
1 ABIN111963 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see IN-05 1
1 ABIN487416 EIA IHC (fro) WB Mouse IgG1 Log in to see HPI-B5
1 ABIN782191 EIA Mouse IgG1 C-Term Log in to see INSC7C9
1 ABIN1107764 EIA IHC (p) Mouse IgG1 Log in to see E2-E3
1 ABIN400749 EIA IHC (p) HRP Mouse IgG1 Log in to see 8-00E-002
1 ABIN113504 EIA IHC (fro) IHC (p) Rabbit Log in to see Polyclonal 1
1 ABIN112204 EIA IHC (fro) Mouse IgG1 Log in to see 5E4-3
1 ABIN112182 ELISA (Capture) EIA Mouse IgG1 Log in to see E6-E5 (7F8)
1 ABIN191769 EIA IHC (fro) IF IHC (p) RIA WB Rabbit AA 57-87 Log in to see Polyclonal
1 ABIN268266 EIA Mouse IgG1 kappa Log in to see D3E7
1 ABIN356107 EIA IHC (fro) Mouse IgG1 Log in to see D4B8

General

Antigen Insulin (INS) Antibodies
Reactivity Human
(949), (397), (284), (274), (262), (121), (49), (6), (6), (5), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(835), (182), (54), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(56), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(622), (371), (247), (233), (204), (204), (161), (138), (70), (66), (40), (27), (16), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN111474) Images
Specificity This monoclonal C-PEP-01 anitbody reacts with the C-peptide of Human Proinsulin. In normal tissues it reacts with normal pancreatic islet Beta-cells. In tumor tissues it stains Insulin secreting neoplasms (insulinomas). The antibody reacts with Human Proinsulin but not with Insulin, Glucagon or Somatostatin, and recognizes the amino acid residues 8-13 and 25-31.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Also reacts to: Proinsulin
Purification Ascites
Immunogen C-Peptide of Human Proinsulin
Clone C-PEP-01
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN111474) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background A precursor of Insulin it is converted to Insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C Peptide are produced. C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN111474) Images back to top
Application Notes Immunohistochemistry on Paraffin Sections: 0.2 μg/mL (1/5000) (No pretreatmentnecessary). Recommended Positive Control: Human pancreas. Has been described to work in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN111474) Images back to top
Reconstitution Restore with 0.5 mL distilled water.
Concentration 1.0 mg/mL
Buffer Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.1 % Kathon as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Insulin antibody (ABIN111474)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991