anti-Rabbit Insulin antibody for Immunohistochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Epitope
AA 1-84
27
24
19
15
12
10
7
6
6
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
965
396
302
282
261
120
49
6
6
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
842
183
54
25
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
57
42
41
28
28
27
23
23
18
17
17
17
17
17
17
16
16
12
11
11
11
11
11
5
5
5
5
5
5
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC)
622
372
247
234
208
205
160
142
71
66
40
28
18
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN3026326
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.870993 ABIN3026546 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04
8.870993 ABIN3025694 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see SPM531
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
1 ABIN3026547 IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04
1 ABIN3026327 IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05
1 ABIN3026328 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05
1 ABIN3026548 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04
1 ABIN1585829 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN3025690 IHC Mouse IgG1, kappa Log in to see IRDN-805
1 ABIN3025695 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see SPM531
1 ABIN2934881 ELISA IF/ICC IHC WB Guinea Pig AA 25-110 Log in to see Polyclonal

General

Antigen Insulin (INS) Antibodies
Epitope AA 1-84
(27), (24), (19), (15), (12), (10), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
(965), (396), (302), (282), (261), (120), (49), (6), (6), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(842), (183), (54), (25), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(57), (42), (41), (28), (28), (27), (23), (23), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (11), (11), (11), (11), (5), (5), (5), (5), (5), (5), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC)
(622), (372), (247), (234), (208), (205), (160), (142), (71), (66), (40), (28), (18), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Characteristics This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.
Purification Protein G affinity chromatography
Immunogen Full length purified pig Insulin (aa 1-84), clones E2-E3 and 2D11-H5, was used as the immunogen for this antibody cocktail.
Clone E2-E3-2D11-H5 or INS04-INS05
Isotype IgG1, kappa

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.
Gene ID 3630
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Insulin (INS) (AA 1-84) antibody (ABIN3026326) IHC testing of human pancreas stained with Insulin antibody cocktail (E2-E3 + 2D11-H5).