|Application / Reactivity||Hormone|
|ELISA||1 ELISA Kits|
|Antigen||Insulin (INS) ELISA Kits|
Kits with alternative reactivity to:
|Detection Range||0-200 μIU/mL|
|Minimum Detection Limit||0 μIU/mL|
|Supplier||Log in to see|
Product Details Insulin ELISA KitTarget details Application Details Handling Images
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|Alternative Name||Insulin (INS ELISA Kit Abstract)|
Insulin is the principal hormone responsible for glucose metabolism. It is synthesized in the cells of the islets of Langerhans as the precuror, proinsulin, which is processed to form C-peptide and insulin and both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chairs, the A chain (21 amino acids) and the B chain (30 amino acids), which are linked by two inter-chain disulphide bridges. There is, in addition, a single intra-chain disulphide bridge in the A chain. The sequence of insulin is highly conserved in mammalian species, and is homologous with the insulin-like growth factors IGF-I and IGF-II. Secretion of insulin is mainly controlled by plasma glucose concentration and the hormones have a number of important metabolic actions. Its principal function is to control the uptake and utilization of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagons, epinephrine(adrenaline), growth hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes (DDM) and some other conditions such as hypopituitarism. Insulin concentrations may be raised in non-insulin- dependant diabetes (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing?s Syndrome and Acromegaly.
The main clinical utility measurement is in the investigation of hypoglycaemia. Insulin assay have been used in the following applications:
1. To assess the residual cell function, especially in newly diagnosed cases of IDDM.
2. As an aid to the discrimination between IDDM and NIDDM.
3. The diagnosis of insulinoma.
4. In the investigation of the pathophysiology of diabetes mellitus.
Insulin assays are the essentials in various dynamic tests, such as oral of intravenous glucose tolerance tests (OGTT and IVGTT), to determine the insulin response of the pancreas and the degree of insulin resistance. In many applications, insulin measurements may be complicated by cross-reactivity with partially degraded insulin, proinsulin and split forms of proinsulin. Immune complexes of these molecules are essentially problematic in patients who have developed anti-insulin antibodies through animal insulin administration.
|Research Area||Neurology, Diabetes, Hormones|
|Pathways||NF-kappaB Signaling, RTK Signaling|
Application DetailsProduct Details Insulin ELISA Kit Target details Handling Images back to top
|Sample Volume||25 μL|
|Assay Time||< 1 h|
|Restrictions||For Research Use only|
HandlingProduct Details Insulin ELISA Kit Target details Application Details Images back to top
|Expiry Date||12-14 months|