Mouse (Murine) Insulin ELISA Kit

Recommended Insulin Product (supplied by: Log in to see )

Antigen
Insulin (INS) ELISA Kits
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
62
32
29
22
15
14
13
13
10
9
8
7
6
3
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
5-400 μIU/mL
Minimum Detection Limit
5 μIU/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1979550
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.70292 ABIN415467 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 123.5-10000 pg/mL 123.5 pg/mL Log in to see 6
9.70292 ABIN366323 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate 15.6-1000 nlU/mL 15.6 nlU/mL Log in to see 10
9.70292 ABIN2703271 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4-300 μIU/mL 4 μIU/mL Log in to see
9.70292 ABIN2703269 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.5-300 μIU/mL 4.5 μIU/mL Log in to see
9.70292 ABIN2669917 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.625-40 MioU/L 0.625 MioU/L Log in to see
1 ABIN1028895 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN1116657 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1057576 Competition ELISA Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma 5.0-100 pM/L 5.0 pM/L Log in to see
1 ABIN773301 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN5591735 Competition ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.625-40 mIU/L 0.625 mIU/L Log in to see
1 ABIN1813715 Sandwich ELISA Body Fluids, Tissue Lysate, Cell Culture Supernatant, Serum Log in to see 3
1 ABIN366374 Serum, Plasma, Tissue Homogenate 3.12-200 pg/mL 3.12 pg/mL Log in to see
1 ABIN5520035 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN773538 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
1 ABIN5520034 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN1774778 Serum, Plasma 0-100 μIU/mL 0 μIU/mL Log in to see 2
1 ABIN5519213 Sandwich ELISA Plasma, Serum, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN1846978 Serum, Plasma, Biological Fluids 0-140 μIU/mL 0 μIU/mL Log in to see
1 ABIN2684753 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 0-140 μIU/mL Log in to see
1 ABIN4991281 Sandwich ELISA Plasma, Serum, Tissue Samples, Biological Fluids Log in to see

Top referenced Insulin ELISA Kit for ELISA

Similar Insulin ELISA Kits

Application / Reactivity Chicken Cow (Bovine) Dog (Canine) Fish Goat Guinea Pig Horse (Equine) Human Monkey Pig (Porcine) Rabbit Rat (Rattus) Rhesus Monkey Rodents
ELISA 9 ELISA Kits 14 ELISA Kits 14 ELISA Kits 13 ELISA Kits 1 ELISA Kits 7 ELISA Kits 13 ELISA Kits 6 ELISA Kits 62 ELISA Kits 10 ELISA Kits 22 ELISA Kits 15 ELISA Kits 32 ELISA Kits 2 ELISA Kits

General

Antigen Insulin (INS) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(62), (32), (29), (22), (15), (14), (13), (13), (10), (9), (8), (7), (6), (3), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 5-400 μIU/mL
Minimum Detection Limit 5 μIU/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details Insulin ELISA Kit

Target details Application Details Handling ProductDetails: References for Insulin Kit (ABIN1979550) Images
Purpose Mouse Insulin ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA antibody pair detects mouse, human, rat, porcine and bovine Insulin.
Sensitivity 5 MioU/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Insulin ELISA Kit Application Details Handling ProductDetails: References for Insulin Kit (ABIN1979550) Images back to top
Antigen
Alternative Name Insulin (INS ELISA Kit Abstract)
Gene ID 16333
UniProt P01325
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details Insulin ELISA Kit Target details Handling ProductDetails: References for Insulin Kit (ABIN1979550) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent C (Item L) should be used for dilution of serum/plasma/culture supernatants. The Mouse Insulin ELISA Kit Protocol 3 Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. 3. Assay Diluent B (Item E) should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL Assay Diluent C (Item L) into Item C vial to prepare a 1,400 µIU/ml standard solution. Dissolve the powder thoroughly by a gentle mix. Add 180 µL Insulin standard (1,400 µIU/ml) from the vial of Item C, into a tube with 450 µL Assay Diluent C to prepare a 400 µIU/ml standard solution. Pipette 250myl Assay Diluent C into each tube. Use the 400 µIU/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Assay Diluent C serves as the zero standard (0 µIU/ml). 250 µL 250 µL 250 µL 250 µL 250myl 180 µL Standard + 450 µL 250 µL 400 200 100 50 25 12.5 6.25 0 µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 The Mouse Insulin ELISA Kit Protocol 4 mL of Wash Buffer Concentrate into deionized or distilled water to yield 400 mL of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B (Item E) into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B (Item E). For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 30 µL of HRP-Streptavidin concentrate into a tube with 12 mL 1x Assay Diluent B to prepare a 400-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or overnight at 4 °C with gentle shaking. 3. Discard Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Assay Precision Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only

Handling

Product Details Insulin ELISA Kit Target details Application Details ProductDetails: References for Insulin Kit (ABIN1979550) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for Insulin Kit (ABIN1979550)

Product Details Insulin ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Eo, Jeon, Lee, Lim: "Brown Alga Ecklonia cava polyphenol extract ameliorates hepatic lipogenesis, oxidative stress, and inflammation by activation of AMPK and SIRT1 in high-fat diet-induced obese mice." in: Journal of agricultural and food chemistry, Vol. 63, Issue 1, pp. 349-59, 2015

Abdel-Latif: "Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation." in: International immunopharmacology, Vol. 29, Issue 2, pp. 607-12, 2015

Images

Product Details Insulin ELISA Kit Target details Application Details Handling ProductDetails: References for Insulin Kit (ABIN1979550) back to top
Supplier Images
ELISA image for Insulin ELISA Kit (INS) (ABIN1979550) Insulin (INS) ELISA Kit