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anti-Cow (Bovine) NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
N-Term
67
52
48
37
24
23
22
22
19
15
14
13
13
13
7
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
473
212
175
34
29
21
8
6
1
1
Host
Rabbit
330
149
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
18
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
1
Application
Western Blotting (WB)
362
183
144
87
85
78
73
34
32
19
6
5
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN2792116
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
0.13398378 ABIN2707586 IC IF IHC WB Rabbit N-Term, pSer32, pSer36 Log in to see Polyclonal
0.13398378 ABIN2707587 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN2707588 IC IF IP IHC WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN926864 WB Rabbit N-Term Log in to see Polyclonal
0.13398378 ABIN926946 WB Rabbit Middle Region Log in to see Polyclonal
0.0011731827 ABIN2957632 IHC WB Rabbit N-Term, pTyr42 Log in to see Polyclonal
0.0011731827 ABIN4324160 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324191 IP WB Allophycocyanin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324192 IP WB DyLight 650 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324193 IP WB DyLight 680 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324194 IP WB DyLight 488 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324196 IP WB DyLight 755 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324197 IP WB PerCP Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324198 IP WB PE Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324199 IP WB DyLight 550 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324200 IP WB DyLight 350 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324201 IP WB DyLight 405 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324202 IP WB DyLight 405LS Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
0.0011731827 ABIN4324203 IP WB Alexa Fluor 405 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Pig (Porcine) Cow (Bovine) Chicken Dog (Canine)
Western Blotting (WB) 356 Antibodies 153 Antibodies 132 Antibodies 30 Antibodies 26 Antibodies 2 Antibodies 22 Antibodies
Intracellular Staining (ICS) 4 Antibodies 2 Antibodies
Immunoprecipitation (IP) 86 Antibodies 49 Antibodies 45 Antibodies 19 Antibodies 19 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope N-Term
(67), (52), (48), (37), (24), (23), (22), (22), (19), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(473), (212), (175), (34), (29), (21), (8), (6), (1), (1)
Host Rabbit
(330), (149), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(18), (14), (13), (13), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (1)
Application Western Blotting (WB)
(362), (183), (144), (87), (85), (78), (73), (34), (32), (19), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN2792116) Images
Sequence FQAAERPQEW AMEGPRDGLK KERLLDDRHD SGLDSMKDEE YEQMVKELQE
Predicted Reactivity Gyps fulvus :100 %,   Chicken :100 %,   Bovine :100 %,   Mouse :100 %,   Mongolian jird :100 %,   Pig :100 %,   Dog :100 %,   Sheep :100 %,   Rat :100 %,   Human :100 %,  
Characteristics This is a rabbit polyclonal antibody against NFKBIA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NFKBIA

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN2792116) Images back to top
Antigen
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Molecular Weight 35 kDa
Gene ID 4792
NCBI Accession NP_065390, NM_020529
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN2792116) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 317 AA

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN2792116) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFKBIA Antibody (ABIN2792116)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Truhlar, Mathes, Cervantes, Ghosh, Komives: "Pre-folding IkappaBalpha alters control of NF-kappaB signaling." in: Journal of molecular biology, Vol. 380, Issue 1, pp. 67-82, 2008

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN2792116) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (N-Term) (ABIN2792116) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (N-Term) antibody