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anti-Human NFKBIA antibody for Enzyme Immunoassay

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
N-Term, Sumoylation Site
50
48
36
29
24
23
22
15
14
13
13
13
6
5
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
368
173
137
17
12
8
6
4
2
1
Host
Rabbit
319
55
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
14
11
9
8
6
6
4
3
3
3
3
3
3
3
3
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
258
140
119
58
51
49
34
19
12
11
5
5
4
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN357736
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008850278 ABIN116524 EIA IHC (fro) WB Rabbit C-Term Log in to see Polyclonal 2
0.0008850278 ABIN265483 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008850278 ABIN303373 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008850278 ABIN121497 EMSA EIA IP WB Sheep Log in to see Polyclonal
0.0008850278 ABIN2727228 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-NFKBIA antibody for Enzyme Immunoassay

Similar anti-NFKBIA Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 141 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 120 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 50 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope N-Term, Sumoylation Site
(50), (48), (36), (29), (24), (23), (22), (15), (14), (13), (13), (13), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(368), (173), (137), (17), (12), (8), (6), (4), (2), (1)
Host Rabbit
(319), (55), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(14), (11), (9), (8), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(258), (140), (119), (58), (51), (49), (34), (19), (12), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN357736) Images
Specificity This antibody is specific to IKB alpha/IKBA (Sumoylation Site).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IKB-alpha around the sumoylation site.
Isotype Ig

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN357736) Images back to top
Antigen
Alternative Name NFKBIA / IKBA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
Molecular Weight 35609 Da
Gene ID 4792, 9606
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN357736) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN357736) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-NFKBIA Antibody (ABIN357736)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Miskolci, Castro-Alcaraz, Nguyen, Vancura, Davidson, Vancurova: "Okadaic acid induces sustained activation of NFkappaB and degradation of the nuclear IkappaBalpha in human neutrophils." in: Archives of biochemistry and biophysics, Vol. 417, Issue 1, pp. 44-52, 2003

Parcellier, Schmitt, Gurbuxani, Seigneurin-Berny, Pance, Chantôme, Plenchette, Khochbin, Solary, Garrido: "HSP27 is a ubiquitin-binding protein involved in I-kappaBalpha proteasomal degradation." in: Molecular and cellular biology, Vol. 23, Issue 16, pp. 5790-802, 2003

Takada, Mukhopadhyay, Kundu, Mahabeleshwar, Singh, Aggarwal et al.: "Hydrogen peroxide activates NF-kappa B through tyrosine phosphorylation of I kappa B alpha and serine phosphorylation of p65: evidence for the involvement of I kappa B alpha kinase and Syk ..." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 24233-41, 2003

Place, Haspeslagh, Giardina: "Induced stabilization of IkappaBalpha can facilitate its re-synthesis and prevent sequential degradation." in: Journal of cellular physiology, Vol. 195, Issue 3, pp. 470-8, 2003

Kim, Lee, Son, Park, Bae: "Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28462-9, 2003

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN357736) back to top
Supplier Images
 image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (N-Term) (ABIN357736) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (N-Term), (Sumoylation Site) antibody