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anti-Human NFKBIA antibody for Flow Cytometry

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
AA 12-41, Ser32, Ser36
68
52
49
37
23
22
22
21
18
15
14
13
13
12
7
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
480
217
181
38
31
23
9
6
2
Host
Rabbit
333
153
5
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
14
13
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
369
188
148
88
85
80
72
35
34
19
6
4
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN652417
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29033512 ABIN252471 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920 32
0.29033512 ABIN1999494 FACS IF FITC Mouse IgG2b AA 2-317 Log in to see 2
0.29033512 ABIN2446908 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.29033512 ABIN3032048 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 12-41 Log in to see Polyclonal
0.0010400229 ABIN208062 FACS IP WB Mouse IgG1, kappa AA 32-291 Log in to see 6A920
0.0010400229 ABIN2664012 FACS Rabbit IgG Log in to see Polyclonal 3
0.0010400229 ABIN4880898 ELISA FACS ICC WB Mouse IgG1 AA 150-291 Log in to see 4D4F2
0.0010400229 ABIN4880897 ELISA FACS ICC IHC WB Mouse IgG1 AA 150-291 Log in to see 4D4C4
0.0010400229 ABIN1811860 FACS IF IHC (p) WB Rabbit AA 19-49 Log in to see Polyclonal
0.0010400229 ABIN4904021 FACS IF IHC IP WB Mouse IgG Log in to see
0.0010400229 ABIN4324163 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010400229 ABIN4904022 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal
0.0010400229 ABIN2289284 FACS IP IHC WB Mouse IgG1 AA 32-391 Log in to see 6D472
0.0010400229 ABIN4224347 FACS WB Biotin Mouse IgG1 CAS3 cleavage site Log in to see 5D1623
0.0010400229 ABIN4224346 FACS WB Allophycocyanin Mouse IgG1 CAS3 cleavage site Log in to see 5D1623
0.0010400229 ABIN4224349 FACS WB PerCP Mouse IgG1 CAS3 cleavage site Log in to see 5D1623
0.0010400229 ABIN4224350 FACS WB PE Mouse IgG1 CAS3 cleavage site Log in to see 5D1623
0.0010400229 ABIN4324171 FACS ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010400229 ABIN4324177 FACS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010400229 ABIN4324173 FACS ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG1 kappa AA 32-291 Log in to see 6A920

Top referenced anti-NFKBIA antibody for Flow Cytometry

Similar anti-NFKBIA Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 188 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 86 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 81 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 147 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 71 Antibodies
Immunoprecipitation (IP) 85 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 12-41, Ser32, Ser36
(68), (52), (49), (37), (23), (22), (22), (21), (18), (15), (14), (13), (13), (12), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(480), (217), (181), (38), (31), (23), (9), (6), (2)
Host Rabbit
(333), (153), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This NFKBIA antibody is un-conjugated
(19), (14), (13), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(369), (188), (148), (88), (85), (80), (72), (35), (34), (19), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) Images
Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from human NFKBIA.
Predicted Reactivity Mouse (Murine),Pig (Porcine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 AA from human NFKBIA.
Clone RB21911
Isotype Ig

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Antigen
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Molecular Weight 35478 DA
Gene ID 4792
UniProt P25963
Research Area Apoptosis/Necrosis, Cell Signaling, Transcription Factors, Cell Structure
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-NFKBIA Antibody (ABIN652417)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Tojima, Fujimoto, Delhase, Chen, Hatakeyama, Nakayama, Kaneko, Nimura, Motoyama, Ikeda, Karin, Nakanishi: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000

Gil, Alcamí, Esteban: "Activation of NF-kappa B by the dsRNA-dependent protein kinase, PKR involves the I kappa B kinase complex." in: Oncogene, Vol. 19, Issue 11, pp. 1369-78, 2000

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Western blot analysis of NFKBIA-S32/36 (ABIN652417) in Hela cell line lysates (35 µg/...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/...
Flow Cytometry (FACS) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histog...