anti-Human NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Epitope
N-Term
71
48
45
39
23
23
22
21
18
18
15
15
14
14
5
5
5
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine)
498
260
215
48
40
29
19
11
2
2
2
1
1
Host
Rabbit
338
165
6
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
15
14
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
386
180
168
89
84
81
66
40
30
19
9
6
5
4
3
3
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2792665
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3471746 ABIN652417 FACS IF IHC (p) WB Rabbit Ig AA 12-41, Ser32, Ser36 Log in to see Polyclonal 2
3.3471746 ABIN1531661 IHC ELISA WB Rabbit IgG pTyr305 Log in to see Polyclonal 1
3.3471746 ABIN1531880 IF IHC ELISA WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 1
3.3471746 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
3.3471746 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
3.3471746 ABIN389074 WB Rabbit Ig AA 1-30 Log in to see Polyclonal 1
3.3471746 ABIN674454 IF (p) IHC (p) WB Rabbit IgG AA 5-34 Log in to see Polyclonal 10
3.3471746 ABIN252471 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920 33
3.3471746 ABIN252664 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 11
3.3471746 ABIN252071 ELISA WB Biotin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 1
3.3471746 ABIN223053 IP IHC WB Mouse IgG1 Log in to see 6A055 1
3.3471746 ABIN314166 ICC IF IHC (p) IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 5
3.3471746 ABIN2668625 WB Mouse IgG1 AA 32-291 Log in to see 6A920 6
3.3471746 ABIN2779315 WB Rabbit N-Term Log in to see Polyclonal
3.3471746 ABIN1882268 WB Mouse IgG1, kappa Log in to see 1121CT8-6-1 4
3.3471746 ABIN2668610 WB Mouse IgG1 Log in to see 39A1431 3
3.3471746 ABIN3043889 IHC (p) WB Rabbit IgG AA 3-112 Log in to see Polyclonal
3.3471746 ABIN967353 IP WB Mouse IgG1 pSer32 Log in to see 39A1413 6
3.3471746 ABIN967354 IP WB Mouse IgG1 AA 105-291 Log in to see 6A920 6
3.3471746 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Pig (Porcine) Human
ELISA 3 Antibodies 180 Antibodies
Flow Cytometry (FACS) 1 Antibodies 84 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 44 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope N-Term
(71), (48), (45), (39), (23), (23), (22), (21), (18), (18), (15), (15), (14), (14), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Pig (Porcine)
(498), (260), (215), (48), (40), (29), (19), (11), (2), (2), (2), (1), (1)
Host Rabbit
(338), (165), (6)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(19), (15), (14), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(386), (180), (168), (89), (84), (81), (66), (40), (30), (19), (9), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792665) Images
Sequence MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ
Cross-Reactivity Human, Pig (Porcine)
Predicted Reactivity Human: 100%, Pig: 76%
Characteristics This is a rabbit polyclonal antibody against NFKBIA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NFKBIA

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792665) Images back to top
Antigen
Alternative Name NFKBIA / IKBA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Alias Symbols: IKBA, MAD-3, NFKBI
Protein Interaction Partner: BTRC, NEDD9, IKBKB, CHUK, UBC, MTOR, IKBKG, SKP1, FBXW11, UBE2D3, RELA, NPLOC4, VCP, UFD1L, NFKB1, TNF, SUMO1, UBE2D1, COPS3, GPS1, SUMO3, Csnk2b, PRKCA, ST7, STAT1, REL, POLR2C, CD7, ARRB2, ARRB1, SUMO2, PSMA1, ZNF212, IKZF4, ATF4, TBK1, IKBKE, DNAJA3, S
Protein Size: 317
Molecular Weight 36 kDa
Gene ID 4792
NCBI Accession NM_020529, NP_065390
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792665) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Antigen size: 317 AA

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details ProductDetails: References for anti-NFKBIA antibody (ABIN2792665) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-NFKBIA antibody (ABIN2792665)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Janssen, van Wengen, Hoeve, ten Dam, van der Burg, van Dongen, van de Vosse, van Tol, Bredius, Ottenhoff, Weemaes, van Dissel, Lankester: "The same IkappaBalpha mutation in two related individuals leads to completely different clinical syndromes." in: The Journal of experimental medicine, Vol. 200, Issue 5, pp. 559-68, 2004

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792665) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (N-Term) (ABIN2792665) WB Suggested Anti-NFKBIA Antibody Titration: 0.2-1 ug/ml
Positive Contro...