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anti-Human NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
pTyr305
51
48
36
29
24
23
21
15
14
13
13
13
7
6
5
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
413
173
137
17
12
8
6
4
2
1
Host
Rabbit
321
98
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
18
13
12
11
6
6
6
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
302
178
118
58
54
51
49
34
19
11
6
5
4
3
2
1
1
1
1
1
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Catalog No. ABIN1531661
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12429275 ABIN1531880 IF IHC ELISA WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 1
0.12429275 ABIN652417 FACS IF IHC (p) WB Rabbit Ig AA 12-41, Ser32, Ser36 Log in to see Polyclonal 2
0.12429275 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
0.12429275 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12429275 ABIN2792665 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.12429275 ABIN389074 WB Rabbit Ig AA 1-30 Log in to see Polyclonal 1
0.12429275 ABIN223053 IP IHC WB Mouse IgG1 Log in to see 6A055 1
0.12429275 ABIN2668625 WB Mouse IgG1 AA 32-291 Log in to see 6A920 4
0.12429275 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.12429275 ABIN968113 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.12429275 ABIN967353 IP WB Mouse IgG1 pSer32 Log in to see 39A1413 6
0.12429275 ABIN967354 IP WB Mouse IgG1 AA 105-291 Log in to see 6A920 6
0.12429275 ABIN2668610 WB Mouse IgG1 Log in to see 39A1431 3
0.12429275 ABIN1882268 WB Mouse IgG1, kappa Log in to see 1121CT8-6-1 4
0.12429275 ABIN1558178 WB Mouse N-Term Log in to see 2E10-B11-F6
0.12429275 ABIN2779315 WB Rabbit N-Term Log in to see Polyclonal
0.12429275 ABIN1532911 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12429275 ABIN1532903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12429275 ABIN2856763 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.12429275 ABIN155061 IP WB Mouse IgG1 Log in to see 6A055

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 41 Antibodies 41 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 4 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 7 Antibodies
Immunofluorescence (IF) 27 Antibodies 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 68 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 94 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope pTyr305
(51), (48), (36), (29), (24), (23), (21), (15), (14), (13), (13), (13), (7), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(413), (173), (137), (17), (12), (8), (6), (4), (2), (1)
Host Rabbit
(321), (98), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(18), (13), (12), (11), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(302), (178), (118), (58), (54), (51), (49), (34), (19), (11), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) Images
Specificity IkappaB-alpha (Phospho-Tyr305) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at Tyr305.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305.
Isotype IgG

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Antigen
Alternative Name IkappaB-alpha (NFKBIA Antibody Abstract)
Background Molecular weight of target protein: 35 kDa
Synonyms: I-kappa-B-alpha, IkappaBalpha, IKBA, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1
Molecular Weight 35 kDa
Gene ID 4792
OMIM 164008
UniProt P25963
Research Area Transcription Factors
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.81328 (NCBI Gene Symbol: NFKBIA)

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NFKBIA Antibody (ABIN1531661)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pTyr305) (ABIN1531661) Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h,...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pTyr305) (ABIN1531661) Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-al...