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anti-Human NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
pTyr305
68
52
49
37
23
22
22
21
18
15
14
13
13
13
7
5
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
480
216
180
38
31
23
9
6
2
Host
Rabbit
333
153
5
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
14
13
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
369
187
147
88
86
81
73
35
34
19
6
4
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN1531661
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1205588 ABIN1531880 IF IHC ELISA WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 1
0.1205588 ABIN652417 FACS IF IHC (p) WB Rabbit Ig AA 12-41, Ser32, Ser36 Log in to see Polyclonal 2
0.1205588 ABIN252471 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920 32
0.1205588 ABIN389074 WB Rabbit Ig AA 1-30 Log in to see Polyclonal 1
0.1205588 ABIN2792665 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.1205588 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
0.1205588 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
0.1205588 ABIN223053 IP IHC WB Mouse IgG1 Log in to see 6A055 1
0.1205588 ABIN252664 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 9
0.1205588 ABIN252071 ELISA WB Biotin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 1
0.1205588 ABIN2668625 WB Mouse IgG1 AA 32-291 Log in to see 6A920 4
0.1205588 ABIN967353 IP WB Mouse IgG1 pSer32 Log in to see 39A1413 6
0.1205588 ABIN967354 IP WB Mouse IgG1 AA 105-291 Log in to see 6A920 6
0.1205588 ABIN968113 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.1205588 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.1205588 ABIN1882268 WB Mouse IgG1, kappa Log in to see 1121CT8-6-1 4
0.1205588 ABIN2668610 WB Mouse IgG1 Log in to see 39A1431 3
0.1205588 ABIN1558178 WB Mouse N-Term Log in to see 2E10-B11-F6
0.1205588 ABIN2779315 WB Rabbit N-Term Log in to see Polyclonal
0.1205588 ABIN1532903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 45 Antibodies 46 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 21 Antibodies 21 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 25 Antibodies
Immunofluorescence (IF) 46 Antibodies 52 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 90 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 46 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 136 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope pTyr305
(68), (52), (49), (37), (23), (22), (22), (21), (18), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(480), (216), (180), (38), (31), (23), (9), (6), (2)
Host Rabbit
(333), (153), (5)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(19), (14), (13), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(369), (187), (147), (88), (86), (81), (73), (35), (34), (19), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) Images
Specificity IkappaB-alpha (Phospho-Tyr305) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at Tyr305.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305.
Isotype IgG

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Antigen
Alternative Name IkappaB-alpha (NFKBIA Antibody Abstract)
Background Molecular weight of target protein: 35 kDa
Synonyms: I-kappa-B-alpha, IkappaBalpha, IKBA, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1
Molecular Weight 35 kDa
Gene ID 4792
OMIM 164008
UniProt P25963
Research Area Transcription Factors
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.81328 (NCBI Gene Symbol: NFKBIA)

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN1531661) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NFKBIA Antibody (ABIN1531661)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531661) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pTyr305) (ABIN1531661) Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h,...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pTyr305) (ABIN1531661) Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-al...