You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Epitope
AA 12-41, Ser32, Ser36
69
49
44
38
23
23
22
20
18
17
15
15
14
14
5
5
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
486
260
216
49
40
29
17
11
2
2
2
1
1
Host
Rabbit
330
161
5
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
15
14
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
375
176
167
83
83
79
65
40
28
19
9
6
5
4
3
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN652417
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0742087 ABIN1531661 IHC ELISA WB Rabbit IgG pTyr305 Log in to see Polyclonal 1
3.0742087 ABIN1531880 IF IHC ELISA WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal 1
3.0742087 ABIN389074 WB Rabbit Ig AA 1-30 Log in to see Polyclonal 1
3.0742087 ABIN2792229 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0742087 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
3.0742087 ABIN252471 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920 32
3.0742087 ABIN2792665 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0742087 ABIN252664 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 10
3.0742087 ABIN223053 IP IHC WB Mouse IgG1 Log in to see 6A055 1
3.0742087 ABIN252071 ELISA WB Biotin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 1
3.0742087 ABIN2668625 WB Mouse IgG1 AA 32-291 Log in to see 6A920 4
3.0742087 ABIN314166 ICC IF IHC (p) IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 5
3.0742087 ABIN1882268 WB Mouse IgG1, kappa Log in to see 1121CT8-6-1 4
3.0742087 ABIN2668610 WB Mouse IgG1 Log in to see 39A1431 3
3.0742087 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
3.0742087 ABIN968113 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
3.0742087 ABIN967353 IP WB Mouse IgG1 pSer32 Log in to see 39A1413 6
3.0742087 ABIN967354 IP WB Mouse IgG1 AA 105-291 Log in to see 6A920 6
3.0742087 ABIN674454 IF (p) IHC (p) WB Rabbit IgG AA 5-34 Log in to see Polyclonal 10
3.0742087 ABIN1558178 WB Mouse N-Term Log in to see 2E10-B11-F6

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 176 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 84 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 80 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 166 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 12-41, Ser32, Ser36
(69), (49), (44), (38), (23), (23), (22), (20), (18), (17), (15), (15), (14), (14), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(486), (260), (216), (49), (40), (29), (17), (11), (2), (2), (2), (1), (1)
Host Rabbit
(330), (161), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This NFKBIA antibody is un-conjugated
(19), (15), (14), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(375), (176), (167), (83), (83), (79), (65), (40), (28), (19), (9), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) Images
Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from human NFKBIA.
Predicted Reactivity Mouse (Murine),Pig (Porcine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 AA from human NFKBIA.
Clone RB21911
Isotype Ig

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Antigen
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Molecular Weight 35478 DA
Gene ID 4792
UniProt P25963
Research Area Apoptosis/Necrosis, Cell Signaling, Transcription Factors, Cell Structure
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN652417) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-NFKBIA Antibody (ABIN652417)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Tojima, Fujimoto, Delhase, Chen, Hatakeyama, Nakayama, Kaneko, Nimura, Motoyama, Ikeda, Karin, Nakanishi: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000

Gil, Alcamí, Esteban: "Activation of NF-kappa B by the dsRNA-dependent protein kinase, PKR involves the I kappa B kinase complex." in: Oncogene, Vol. 19, Issue 11, pp. 1369-78, 2000

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN652417) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Western blot analysis of NFKBIA-S32/36 (ABIN652417) in Hela cell line lysates (35 µg/...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/...
Flow Cytometry (FACS) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 12-41) (ABIN652417) Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histog...