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anti-Mouse (Murine) NFKBIA antibody for Immunocytochemistry

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Epitope
AA 32-291
69
50
44
39
24
24
22
22
19
18
15
15
14
14
5
5
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
496
266
221
49
40
29
17
11
2
2
2
1
1
Host
Mouse
341
160
5
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
15
14
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
384
185
172
83
83
80
65
40
28
20
7
6
5
3
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN252471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN314166 ICC IF IHC (p) IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal 5
1 ABIN2856763 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN4324166 ICC IF IHC IHC (p) WB Rabbit pSer32, pSer36 Log in to see Polyclonal
1 ABIN2448340 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4324163 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324207 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324208 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324177 FACS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324173 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324172 FACS ICC IF IHC IHC (p) WB DyLight 650 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324210 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324174 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324176 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324180 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324181 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324179 FACS ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4324209 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN4995451 FACS ICC IF IHC (p) FITC Mouse IgG1 kappa AA 32-291 Log in to see 6A920
1 ABIN2743228 ICC IF IHC (p) WB Rabbit Ser36, pSer32 Log in to see Polyclonal
1 ABIN2626076 ICC IF IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-NFKBIA antibody for Immunocytochemistry

Similar anti-NFKBIA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
ELISA 58 Antibodies 60 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 21 Antibodies 22 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 97 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 165 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 32-291
(69), (50), (44), (39), (24), (24), (22), (22), (19), (18), (15), (15), (14), (14), (5), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(496), (266), (221), (49), (40), (29), (17), (11), (2), (2), (2), (1), (1)
Host Mouse
(341), (160), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is un-conjugated
(19), (15), (14), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(384), (185), (172), (83), (83), (80), (65), (40), (28), (20), (7), (6), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN252471) Images
Specificity A 40 kDa band is observed.
Purification Protein G purified
Immunogen A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Clone 6A920
Isotype IgG1 kappa

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN252471) Images back to top
Antigen
Alternative Name IkB-alpha (NFKBIA Antibody Abstract)
Background Gene Symbol: NFKBIA
Gene ID 4792
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN252471) Images back to top
Application Notes Western Blot 1-2 μg/mL, Simple Western 1:20, Flow Cytometry 0.25-1 μg/10^6 cells, Immunohistochemistry 1:20-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:1000, Immunoprecipitation 1 μg/mL, Immunohistochemistry-Paraffin 1:100, CyTOF-readyIn Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN252471) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-NFKBIA Antibody (ABIN252471)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Gao, Liu, Wu, Zhao, Wang, Gao, Wen, Zhang, Guo, Zhou, Yuan: "IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 347-58, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Tomasi, Ramani, Ryoo: "Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response." in: The American journal of pathology, Vol. 186, Issue 9, pp. 2326-36, 2016 Further details: Western Blotting

Hochrainer, Pejanovic, Olaseun, Zhang, Iadecola, Anrather: "The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9889-904, 2015 (Sample species: Human). Further details: Western Blotting

Ikeno, Apel, Zouboulis, Luger, Böhm: "L-Ascorbyl-2-phosphate attenuates NF-?B signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion." in: Archives of dermatological research, Vol. 307, Issue 7, pp. 595-605, 2015 (Sample species: Human). Further details: Western Blotting

Tabit, Shenouda, Holbrook, Fetterman, Kiani, Frame, Kluge, Held, Dohadwala, Gokce, Farb, Rosenzweig, Ruderman, Vita, Hamburg: "Protein kinase C-β contributes to impaired endothelial insulin signaling in humans with diabetes mellitus." in: Circulation, Vol. 127, Issue 1, pp. 86-95, 2013 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Yang, Zhao, Liu, Hu, Sun, Chang, Wei, Fan, Han: "Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 728020, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Pejanovic, Hochrainer, Liu, Aerne, Soares, Anrather: "Regulation of nuclear factor κB (NF-κB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT)." in: PLoS ONE, Vol. 7, Issue 7, pp. e42020, 2012

Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010

Yu, Su, Zou, Liu, Wu, Kong, Zhuang, Sun, Liu, Hu, Li, Strominger, Zang, Pei, Ge: "An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells." in: Nature immunology, Vol. 9, Issue 8, pp. 898-907, 2008 (Sample species: Mouse (Murine)).

Nishioka, Ikezoe, Jing, Umezawa, Yokoyama et al.: "DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type ..." in: Immunology, Vol. 124, Issue 2, pp. 198-205, 2008

Shakibaei, John, Schulze-Tanzil, Lehmann, Mobasheri et al.: "Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment ..." in: Biochemical pharmacology, Vol. 73, Issue 9, pp. 1434-45, 2007

Endo, Nishio, Enzler, Cottam, Fukuda, James, Karin, Kipps: "BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway." in: Blood, Vol. 109, Issue 2, pp. 703-10, 2007 (Sample species: Human). Further details: Western Blotting

Vilimas, Mascarenhas, Palomero, Mandal, Buonamici, Meng, Thompson, Spaulding, Macaroun, Alegre, Kee, Ferrando, Miele, Aifantis: "Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia." in: Nature medicine, Vol. 13, Issue 1, pp. 70-7, 2007 Further details: Western Blotting,Immunoprecipitation

Perez-Moreno, Davis, Wong, Pasolli, Reynolds, Fuchs: "p120-catenin mediates inflammatory responses in the skin." in: Cell, Vol. 124, Issue 3, pp. 631-44, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting

Chang, Kamata, Solinas, Luo, Maeda, Venuprasad, Liu, Karin: "The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover." in: Cell, Vol. 124, Issue 3, pp. 601-13, 2006 Further details: Western Blotting

Strozyk, Pöppelmann, Schwarz, Kulms: "Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome." in: Oncogene, Vol. 25, Issue 47, pp. 6239-51, 2006 (Sample species: Human). Further details: Western Blotting

Macagno, Molteni, Rinaldi, Bertoni, Lanzavecchia, Rossetti, Sallusto: "A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression." in: The Journal of experimental medicine, Vol. 203, Issue 6, pp. 1481-92, 2006 Further details: Western Blotting

Santhi, Sebastian, Varghese, Prakash, Pillai: "NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins." in: Experimental and molecular pathology, Vol. 81, Issue 2, pp. 123-30, 2006

Ishiguro, Ando, Maeda, Hasegawa, Kadomatsu, Ohmiya, Niwa, Xavier, Goto: "Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation." in: Toxicology and applied pharmacology, Vol. 217, Issue 1, pp. 35-42, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN252471) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucina...
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Western blot analysis of IkBa using ABIN252471 at 2 ug/ml in (A) Daudi and (B) NIH 3T...
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Western Blot: IkB-alpha Antibody (6A920) [ABIN2524717] - Jurkat cells were treated fo...
Simple Western (SimWes) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Simple Western: IkB-alpha Antibody (6A920) [ABIN2524717] - Simple Western lane view s...
Flow Cytometry (FACS) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Flow Cytometry: IkB-alpha Antibody (6A920) [ABIN2524717] - Intracellular staining of ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 32-291) (ABIN252471) Immunohistochemistry-Paraffin: IkB-alpha Antibody (6A920) [ABIN2524717] - Formalin-fi...