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anti-Mouse (Murine) NFKBIA antibody for Immunocytochemistry

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
Center
51
48
36
29
24
23
22
15
14
13
13
13
7
5
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
368
172
137
17
12
8
6
4
2
1
Host
Rabbit
319
55
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
14
11
9
8
6
6
4
3
3
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
258
140
119
57
51
48
34
19
12
10
6
5
4
3
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2856763
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010134187 ABIN2448340 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.0010134187 ABIN1844549 ICC IF IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal
0.0010134187 ABIN314166 ICC IF IHC (p) IHC WB Rabbit Ser32, Ser36 Log in to see Polyclonal
0.0010134187 ABIN2987541 ICC IF IHC WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal
0.0010134187 ABIN2989427 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010134187 ABIN2857769 ICC IF IHC WB Rabbit pSer32, pSer36 Log in to see Polyclonal

Similar anti-NFKBIA Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 40 Antibodies 141 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 6 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 72 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 114 Antibodies
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope Center
(51), (48), (36), (29), (24), (23), (22), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(368), (172), (137), (17), (12), (8), (6), (4), (2), (1)
Host Rabbit
(319), (55), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(14), (11), (9), (8), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(258), (140), (119), (57), (51), (48), (34), (19), (12), (10), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to IKB alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)
IKB alpha antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.
Isotype IgG

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKB alpha (NFKBIA Antibody Abstract)
Background NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Cytoplasm , Nucleus
Molecular Weight 36 kDa
Gene ID 4792
Research Area Immunology, Innate Immunity
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
Comment

Positive Control: HepG2 , BCL-1 , C2C12 , *MCF7 , *RAW264.7, *B16F10, *HT29

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (Center) (ABIN2856763) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ikappa...
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (Center) (ABIN2856763) WB Image IKB alpha antibody detects IKB alpha protein by Western blot analysis. A. 30...
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (Center) (ABIN2856763) WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 10% SDS PAGE antibody dilute...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (Center) (ABIN2856763) IHC-P Image IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by...