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anti-Viper NGFB antibody for Enzyme Immunoassay

Recommended NGFB Antibody (supplied by: Log in to see )

Antigen
Nerve Growth Factor beta (NGFB) Antibodies
  • Beta-NGF
  • beta-NGF
  • beta-ngf
  • HSAN5
  • hsan5
  • ngfb
  • NGFB
  • Ngfb
  • ns:zft386
  • xx:zft386
  • zgc:109730
Reactivity
Cow (Bovine), Human, Mouse (Murine), Viper
132
83
50
8
3
3
Host
Mouse
118
53
10
4
2
2
Clonality
Monoclonal
Conjugate
This NGFB antibody is conjugated to Biotin
18
8
7
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
124
116
54
25
18
12
11
10
6
4
3
2
2
1
1
Supplier
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Catalog No. ABIN264352
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008858215 ABIN264353 EIA Func IP Mouse IgG1 Log in to see AS18
0.0008858215 ABIN264354 EIA Func Mouse IgG1 Log in to see AS21
0.0008858215 ABIN264355 EIA Func Mouse IgG1 Log in to see AS22

Similar anti-NGFB Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Viper
Western Blotting (WB) 72 Antibodies 44 Antibodies 1 Antibodies
Neutralization (Neut) 25 Antibodies 4 Antibodies 2 Antibodies
Inhibition Assay (InhA) 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 34 Antibodies 30 Antibodies
Immunofluorescence (IF) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies

General

Antigen Nerve Growth Factor beta (NGFB) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Viper
(132), (83), (50), (8), (3), (3)
Host Mouse
(118), (53), (10), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NGFB antibody is conjugated to Biotin
(18), (8), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(124), (116), (54), (25), (18), (12), (11), (10), (6), (4), (3), (2), (2), (1), (1)
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Product Details anti-NGFB Antibody

Target Details NGFB Application Details Handling Images
Specificity Recognizes human, bovine, viper and mouse 2.5S, 7S and bNGF. The epitope has been localized to the C-terminus of hNGF.
Characteristics Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor
Purification Protein G Chromatography
Purity > 95 % by SDS-PAGE
Clone AS18
Isotype IgG1

Target Details NGFB

Product Details anti-NGFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name NGF (NGFB Antibody Abstract)
Background Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90 % homology between the two species. Recently, it has been shown that cells which do not normally respond to NGF only express low affinity receptors from transfected NGF genes but cells which normally are NGF-responsive express high affinity receptors from the same NGF sequences. This supports the hypothesis that the high affinity receptors have a unique component in addition to the subunit common to the low affinity receptors.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB
Gene ID 4803
UniProt P01138
Research Area Neurology, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-NGFB Antibody Target Details NGFB Handling Images back to top
Application Notes Elisa: This antibody has been used as a solution phase antibody at 2 μg/mL in a two siteELISA with unconjugated NGF antibody (ABIN264354 or ABIN264355) for mouse NGFand AS22 for human NGF. Typically for human NGF, the ELISA has a working range of 0-15 ng/mL and a sensitivity of 15 pg/mL with the appropriate standard. Recommended samplevolumes are 25-200 l. Recovery of human NGF in human plasma is typically > 90 % in theELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. (Kenney, JS, Masada,MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NGFB Antibody Target Details NGFB Application Details Images back to top
Buffer PBS containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months