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|Application / Reactivity||Human||Mouse (Murine)||Cow (Bovine)||Viper|
|Western Blotting (WB)||68 Antibodies||41 Antibodies||1 Antibodies|
|Neutralization (Neut)||25 Antibodies||5 Antibodies||2 Antibodies|
|Inhibition Assay (InhA)||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies||3 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||32 Antibodies||29 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||3 Antibodies|
|Antigen||Nerve Growth Factor beta (NGFB) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Viper Alternatives|
|Conjugate||This NGFB antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA)
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Product Details anti-NGFB AntibodyTarget Details NGFB Application Details Handling Images
|Specificity||Recognizes human, bovine, viper and mouse 2.5S, 7S and bNGF. The epitope has been localized to the C-terminus of hNGF.|
|Characteristics||Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor|
|Purification||Protein G Chromatography|
|Purity||> 95 % by SDS-PAGE|
Target Details NGFBProduct Details anti-NGFB Antibody Application Details Handling Images back to top
|Alternative Name||NGF (NGFB Antibody Abstract)|
|Background||Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90 % homology between the two species. Recently, it has been shown that cells which do not normally respond to NGF only express low affinity receptors from transfected NGF genes but cells which normally are NGF-responsive express high affinity receptors from the same NGF sequences. This supports the hypothesis that the high affinity receptors have a unique component in addition to the subunit common to the low affinity receptors.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB|
|Research Area||Neurology, Growth Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling|
Application DetailsProduct Details anti-NGFB Antibody Target Details NGFB Handling Images back to top
Elisa: This antibody has been used as a solution phase antibody at 2 μg/mL in a two siteELISA with unconjugated NGF antibody (ABIN264354 or ABIN264355) for mouse NGFand AS22 for human NGF. Typically for human NGF, the ELISA has a working range of 0-15 ng/mL and a sensitivity of 15 pg/mL with the appropriate standard. Recommended samplevolumes are 25-200 l. Recovery of human NGF in human plasma is typically > 90 % in theELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. (Kenney, JS, Masada,MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NGFB Antibody Target Details NGFB Application Details Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|