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anti-Human PKC zeta antibody for Immunocytochemistry

Recommended PKC zeta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, zeta (PRKCZ) Antibodies
  • 14-3-3-zetaisoform
  • AI098070
  • aPKCzeta
  • C80388
  • pkc-zeta
  • PKC-ZETA
  • pkc2
  • PKC2
  • pkcz
  • Pkcz
  • PKCzeta
  • PRKCZ
  • prkcz-A
  • r14-3-3
  • R74924
  • si:ch211-150o23.4
  • TPKC
  • zetaPKC
Epitope
Thr410
46
38
17
14
11
7
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
158
92
76
5
4
2
2
2
1
1
Host
Rabbit
186
4
1
Clonality
Monoclonal
Conjugate
This PKC zeta antibody is un-conjugated
8
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
126
91
87
30
25
11
10
9
4
3
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN256888
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN258979 ICC IF IHC (p) IHC WB Rabbit IgG Thr560 Log in to see EP2037AY
0.0010161072 ABIN1860239 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 404-591 Log in to see Polyclonal
0.0010161072 ABIN2884235 ICC IHC ELISA WB Rabbit IgG pThr410 Log in to see Polyclonal
0.0010161072 ABIN1844816 ICC IF IHC WB Rabbit pThr410 Log in to see Polyclonal
0.0010161072 ABIN2627214 ICC Rabbit C-Term Log in to see Polyclonal
0.0010161072 ABIN1844818 ICC IF IHC WB Rabbit Log in to see Polyclonal
0.0010161072 ABIN1847212 ICC IF IHC ELISA WB Rabbit Internal Region, pThr410 Log in to see Polyclonal
0.0010161072 ABIN2858176 ICC IF IHC WB Rabbit pThr410 Log in to see Polyclonal
0.0010161072 ABIN2859008 ICC IF IHC WB Rabbit Log in to see Polyclonal

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General

Antigen Protein Kinase C, zeta (PRKCZ) Antibodies
Epitope Thr410
(46), (38), (17), (14), (11), (7), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (92), (76), (5), (4), (2), (2), (2), (1), (1)
Host Rabbit
(186), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC zeta antibody is un-conjugated
(8), (7), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(126), (91), (87), (30), (25), (11), (10), (9), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-PKC zeta Antibody

Target Details PKC zeta Application Details Handling Images
Specificity This antibody detects PKC zeta/lambda phosphorylated at T410/412.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Tissue culture supernatant
Immunogen A phospho specific peptide corresponding to residues surrounding Threonine 410 and 412 of human PKC zeta/lambda.
Clone EP1491Y
Isotype IgG

Target Details PKC zeta

Product Details anti-PKC zeta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCZ (PRKCZ Antibody Abstract)
Background The atypical protein kinase C isoform, PKCzeta, has been implicated in the control ofextracellular signal-regulated kinase (ERK) and nuclear factor (NF)-kappa B pathways.It has been shown that the loss of PKCzeta selectively impairs signaling through theB-cell receptor, resulting in inhibition of cell proliferation and survival, as well as defectsin the activation of ERK and the transcription of NF-kappa B-dependent genes (1). Thelack of PKCzeta in embryonic fibroblasts (EFs) severely impairs kappaB-dependenttranscriptional activity as well as cytokine-induced phosphorylation of p65. Also, acytokine-inducible interaction of PKCzeta with p65 was detected which requires theprevious degradation of IkappaB (2). It has been found that persistent PKCzeta activitymaintains potentiated responses, not only of the strongly tetanized pathway, but also ofthe weakly tetanized pathway. In contrast, an independent, nontetanized pathway wasunaffected by the inhibitor, indicating that the function of PKCzeta was specific to thetagged synapses (3). Alternate Names: Anti-nPKC-zeta antibody.
Gene Symbol: PRKCZ
Gene ID 5590
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-PKC zeta Antibody Target Details PKC zeta Handling Images back to top
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000
Restrictions For Research Use only

Handling

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PKC zeta Antibody Target Details PKC zeta Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (Thr410) (ABIN256888) Western blot analysis on 293 cell lysates using anti-Phospho-PKC zeta/lambda (pT410/4...
Immunohistochemistry (IHC) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (Thr410) (ABIN256888) Immunohistochemical analysis of paraffin-embedded human brain using anti-Phospho-PKC ...
Immunofluorescence (IF) image for anti-PKC zeta antibody (Protein Kinase C, zeta) (Thr410) (ABIN256888) Immunofluorescent staining of HeLa cells using anti-Phospho-PKC zeta/lambda (pT410/40...