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|Application / Reactivity||Chicken|
|ELISA||5 ELISA Kits|
|Antigen||Tumor Necrosis Factor (TNF) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Competition ELISA|
|Detection Range||0.27-200 pg/mL|
|Minimum Detection Limit||0.27 pg/mL|
|2 references available|
|Supplier||Log in to see|
Product Details Tumor Necrosis Factor ELISA KitTarget details Application Details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images
|Purpose||For the quantitative determination of chicken tumor necrosis factor alpha (TNF-alpha) concentrations in serum, plasma, tissue homogenates.|
|Sample Type||Serum, Plasma, Tissue Homogenate|
|Specificity||This assay has high sensitivity and excellent specificity for detection of chicken TNF-alpha.|
|Cross-Reactivity (Details)||Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.|
The sensitivity of this assay, or Lower Limit of Detection (LLD), was defined as the lowest protein concentration that could be differentiated from zero. It was determined as the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
|Material not included||
Target detailsProduct Details Tumor Necrosis Factor ELISA Kit Application Details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
|Alternative Name||Tumor necrosis factor alpha (TNF-alpha) (TNF ELISA Kit Abstract)|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details Tumor Necrosis Factor ELISA Kit Target details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
Detection wavelength: 450 nm
|Sample Volume||50 μL|
|Assay Time||1 - 4.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNF-alpha. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to TNF-alpha. The competitive inhibition reaction is launched between with pre-coated TNF-alpha and TNF-alpha in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TNF-alpha in the sample. The color development is stopped and the intensity of the color is measured.|
Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
|Restrictions||For Research Use only|
HandlingProduct Details Tumor Necrosis Factor ELISA Kit Target details Application Details References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
|Precaution of Use||The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.|
|Storage||4 °C/-20 °C|
|Storage Comment||May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.|
|Expiry Date||6 months|
References for Tumor Necrosis Factor Kit (ABIN364738)Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Wang, Zeng, Wang et al.: "The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by Clostridium perfringens in broilers." in: Journal of animal science, Vol. 93, Issue 10, pp. 4750-60, 2015 (PubMed).
Zhong, Shi, Chen et al.: "Polyphenol extracts from Punica granatum and Terminalia chebula are anti-inflammatory and increase the survival rate of chickens challenged with Escherichia coli." in: Biological & pharmaceutical bulletin, Vol. 37, Issue 10, pp. 1575-82, 2014 (PubMed).