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Chicken Tumor Necrosis Factor ELISA Kit

Recommended Tumor Necrosis Factor Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) ELISA Kits
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Chicken
Alternatives
Kits with alternative reactivity to:
85
65
51
18
12
12
12
10
10
8
6
6
4
3
2
1
1
Methode Type
Competition ELISA
Detection Range
0.27-200 pg/mL
Minimum Detection Limit
0.27 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN364738
$ 865.33
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012092679 ABIN1059618 Sandwich ELISA Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma 50-1000 pg/mL 50 pg/mL Log in to see
0.0012092679 ABIN1567745 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012092679 ABIN1059413 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see
0.0012092679 ABIN2540066 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1-100 pg/mL 1 pg/mL Log in to see

Top referenced Tumor Necrosis Factor ELISA Kit for ELISA

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Application / Reactivity Chicken
ELISA 5 ELISA Kits

General

Antigen Tumor Necrosis Factor (TNF) ELISA Kits
Reactivity Chicken
Kits with alternative reactivity to:
(85), (65), (51), (18), (12), (12), (12), (10), (10), (8), (6), (6), (4), (3), (2), (1), (1)
Methode Type Competition ELISA
Detection Range 0.27-200 pg/mL
Minimum Detection Limit 0.27 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details Tumor Necrosis Factor ELISA Kit

Target details Application Details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images
Purpose For the quantitative determination of chicken tumor necrosis factor alpha (TNF-alpha) concentrations in serum, plasma, tissue homogenates.
Sample Type Serum, Plasma, Tissue Homogenate
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of chicken TNF-alpha.
Cross-Reactivity (Details) Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
Sensitivity 0.27 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD), was defined as the lowest protein concentration that could be differentiated from zero. It was determined as the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Material not included
  • Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
  • An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
  • Squirt bottle, manifold dispenser or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100mL and 500mL graduated cylinders.
  • Deionized or distilled water.
  • Pipettes and pipette tips.
  • Test tubes for dilution.

Target details

Product Details Tumor Necrosis Factor ELISA Kit Application Details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
Antigen
Alternative Name Tumor necrosis factor alpha (TNF-alpha) (TNF ELISA Kit Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details Tumor Necrosis Factor ELISA Kit Target details Handling References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
Application Notes
  • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
  • Grossly hemolyzed samples are not suitable for use in this assay.
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
  • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
  • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
  • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Comment

Detection wavelength: 450 nm

Information on standard material:
Some standards are from natural resource, some are recombinant protein, but the recombinant protein will not expressed from Baculovirus. Generally it's CHO cells, but that depends.The formulation of auxiliary material in the standard is classified, but it dosen't contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
Most of the stop solution are 1 N H2SO4, a few is not. The formulation of wash solution is classified. None of the components contains (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. Some could contain BSA. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The provided antibodies and their host vary in different kits. Some are affinity purified, some are Protein A or G purified

Sample Volume 50 μL
Assay Time 1 - 4.5 h
Plate Pre-coated,Strips (12 x 8)
Protocol This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNF-alpha. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to TNF-alpha. The competitive inhibition reaction is launched between with pre-coated TNF-alpha and TNF-alpha in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TNF-alpha in the sample. The color development is stopped and the intensity of the color is measured.
Sample Collection
  • Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
  • Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
  • Tissue Homogenates: Rinse 100mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20°C. After two freeze-thaw cycles to break the cell membranes, centrifuge the homogenates for 5 minutes at 5000 × g, 2-8°C. Remove and assay the supernate immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Assay Precision Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
  • Intra-assay: CV% less than 8%
  • Inter-assay: CV% less than 10%
Restrictions For Research Use only

Handling

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details References for Tumor Necrosis Factor Kit (ABIN364738) Images back to top
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Handling Advice
  • The kit should not be used beyond the expiration date on the kit label.
  • Do not mix or substitute reagents with those from other lots or sources.
  • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
  • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
  • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
Storage 4 °C/-20 °C
Storage Comment May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.
Expiry Date 6 months

References for Tumor Necrosis Factor Kit (ABIN364738)

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Wang, Zeng, Wang, Zhu, Peng, Hou, Thacker, Qiao: "The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by Clostridium perfringens in broilers." in: Journal of animal science, Vol. 93, Issue 10, pp. 4750-60, 2015

Zhong, Shi, Chen, Xu, Wang, Beier, Hou, Liu: "Polyphenol extracts from Punica granatum and Terminalia chebula are anti-inflammatory and increase the survival rate of chickens challenged with Escherichia coli." in: Biological & pharmaceutical bulletin, Vol. 37, Issue 10, pp. 1575-82, 2014