Mouse (Murine) Tumor Necrosis Factor ELISA Kit

Recommended Tumor Necrosis Factor Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) ELISA Kits
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
87
67
52
21
15
13
12
10
10
10
9
7
4
3
3
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
60-6000 pg/mL
Minimum Detection Limit
60 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625182
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.499232 ABIN2506961 Sandwich ELISA Serum, Plasma 49.2-12000 pg/mL 49.2 pg/mL Log in to see
12.499232 ABIN365457 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 22
12.499232 ABIN415537 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1.000 pg/mL 15.6 pg/mL Log in to see 37
12.499232 ABIN2472103 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.63-1000 pg/mL 15.63 pg/mL Log in to see
12.499232 ABIN411363 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 15.6-1000 pg/mL 15.6 pg/mL Log in to see 113
12.499232 ABIN1572787 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
12.499232 ABIN924831 Sandwich ELISA Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
12.499232 ABIN1117651 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.499232 ABIN4948107 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.499232 ABIN4948106 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.499232 ABIN491829 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 2.74-2.000 pg/mL 2.74 pg/mL Log in to see 3
12.499232 ABIN2708610 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.499232 ABIN2708609 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.499232 ABIN1888505 Sandwich ELISA Plasma, Cell Culture Cells 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.499232 ABIN2746116 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 47-3000 pg/mL 47 pg/mL Log in to see
12.499232 ABIN2115125 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
12.499232 ABIN1305315 Sandwich ELISA Log in to see
1 ABIN456701 Sandwich ELISA Serum, Plasma, Biological Fluids 7.8-500 pg/mL 7.8 pg/mL Log in to see 2
1 ABIN3032845 Competition ELISA Cell Culture Supernatant, Plasma, Serum 93.8-6000 pg/mL 93.8 pg/mL Log in to see
1 ABIN612749 Sandwich ELISA Plasma, Cell Culture Supernatant 15 pg/mL Log in to see 8

General

Antigen Tumor Necrosis Factor (TNF) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(87), (67), (52), (21), (15), (13), (12), (10), (10), (10), (9), (7), (4), (3), (3), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 60-6000 pg/mL
Minimum Detection Limit 60 pg/mL
Application ELISA
Pubmed 20 references available
Supplier Log in to see

Product Details Tumor Necrosis Factor ELISA Kit

Target details Application Details Handling ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182) Images
Purpose Mouse TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 60 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Tumor Necrosis Factor ELISA Kit Application Details Handling ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182) Images back to top
Antigen
Alternative Name TNF-alpha (TNF ELISA Kit Abstract)
Background Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a)
Gene ID 21926
UniProt P06804
Research Area Virology, Atherosclerosis, Immunology, Receptors, Cytokines, Growth Factors, Innate Immunity, Cancer, Cardiovascular, Inflammation
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details Tumor Necrosis Factor ELISA Kit Target details Handling ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 120 µL TNF-alpha standard from the vial of Item C, into a tube with 880 µL Assay Diluent A or 1x Assay Diluent B to prepare a 6,000 pg/mL stock standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 300 µL 300 µL 300 µL 300 µL 300 µL 120 µL standard + 880 µL 300myl 6,000 3,000 1,500 750 375 187.5 93.75 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 300-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 40 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 300-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Mouse TNF-alpha concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 Assay Buffer A 10 100 1,000 10,000 Mouse TNF-alpha concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 Assay Buffer B 10 100 1,000 10,000
Sensitivity: The minimum detectable dose of TNF-alpha is typically less than 60 pg/mL.
Recovery: Recovery was determined by spiking various levels of Mouse TNF-alpha into Mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 94.18 82-103 Plasma 93.82 83-102 Cell culture media 92.46 84-104
Linearity: Sample Type Serum Plasma Cell culture media 1:2 Average % of Expected 93 94 92 Range ( %) 84-102 83-103 82-103 1:4 Average % of Expected 94 95 96 Range ( %) 82-103 84-104 83-104
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182)

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Yu, Yuan, Liu, Zhang, Wang, Zhang, Liu: "Delayed Administration of WP1066, an STAT3 Inhibitor, Ameliorates Radiation-Induced Lung Injury in Mice." in: Lung, Vol. 194, Issue 1, pp. 67-74, 2016

Singh, Kakalij, Kshirsagar, Kumar, Komakula, Diwan: "Cognitive effects of vanillic acid against streptozotocin-induced neurodegeneration in mice." in: Pharmaceutical biology, Vol. 53, Issue 5, pp. 630-6, 2015

Štofilová, Szabadosová, Hr?ková, Salaj, Bertková, Hijová, Strojný, Bomba: "Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine." in: International immunopharmacology, Vol. 24, Issue 2, pp. 361-8, 2015

Dey, Majhi, Mahanti, Dey, Bishayi: "In Vitro Anti-inflammatory and Immunomodulatory Effects of Ciprofloxacin or Azithromycin in Staphylococcus aureus-Stimulated Murine Macrophages are Beneficial in the Presence of Cytochalasin D." in: Inflammation, Vol. 38, Issue 3, pp. 1050-69, 2015

Ahmad, Attia, Bakheet, Zoheir, Ansari, Korashy, Abdel-Hamied, Ashour, Abd-Allah et al.: "Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-?b, STAT3 and pro-inflammatory mediators and enhancement of I?B? and anti-inflammatory ..." in: Inflammation, Vol. 38, Issue 2, pp. 846-57, 2015

Bishayi, Bandyopadhyay, Majhi, Adhikary: "Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophages and possible mechanisms of CCR-2/TLR-2 and MCP-1 signalling during Staphylococcus aureus infection." in: Immunobiology, Vol. 220, Issue 3, pp. 350-62, 2015

Ghosh, Bishayi: "Characterization of Toll-like receptor-4 (TLR-4) in the spleen and thymus of Swiss albino mice and its modulation in experimental endotoxemia." in: Journal of immunology research, Vol. 2015, pp. 137981, 2015

Yu, Yao, He, Tian: "Protection of MPTP-induced neuroinflammation and neurodegeneration by rotigotine-loaded microspheres." in: Life sciences, Vol. 124, pp. 136-43, 2015

Ghosh, Prakash, Manna, Bishayi: "Presence of toll like receptor-2 in spleen, lymph node and thymus of Swiss albino mice and its modulation by Staphylococcus aureus and bacterial lipopolysaccharide. ." in: Indian journal of experimental biology, Vol. 53, Issue 2, pp. 82-92, 2015

Kumar, Sahu, Kumar, Subuddhi, Maji, Jana, Gupta, Raffetseder, Lerm, Ghosh, van Loo, Beyaert, Gupta, Kundu, Basu: "MicroRNA let-7 modulates the immune response to Mycobacterium tuberculosis infection via control of A20, an inhibitor of the NF-?B pathway." in: Cell host & microbe, Vol. 17, Issue 3, pp. 345-56, 2015

Dey, Chaudhuri: "Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes." in: Cytotechnology, 2015

Ghosh, Bishayi et al.: "Toll-like receptor 2 and 6 interdependency in the erosive stage of Staphylococcus aureus induced septic arthritis mediated by IFN-? and IL-6--A possible involvement of IL-17 in the progression of the ..." in: Immunobiology, Vol. 220, Issue 7, pp. 910-23, 2015

Zhong, Xu, Wang, Wang, Guan, Li, He, Liu, Zhou, Li, Wang, Liao: "Identification of anti-inflammatory constituents from Kalimeris indica with UHPLC-ESI-Q-TOF-MS/MS and GC-MS." in: Journal of ethnopharmacology, Vol. 165, pp. 39-45, 2015

Singla, Singla, Abdelli, Glass: "Fibroblast growth factor-9 enhances M2 macrophage differentiation and attenuates adverse cardiac remodeling in the infarcted diabetic heart." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120739, 2015

Li, Chai, Zhang, Li, Wang, Qiu, Liu: "Mycobacterium tuberculosis Mce3E suppresses host innate immune responses by targeting ERK1/2 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3756-67, 2015

Jia, Zheng, Wang, Zhou, Cai, Jia, Yang, Dong, Zhu, Su, Hu: "SIRT1 is a regulator in high glucose-induced inflammatory response in RAW264.7 cells." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120849, 2015

Galam, Parthasarathy, Cho, Cho, Lee, Lockey, Kolliputi: "Adenovirus-mediated transfer of the SOCS-1 gene to mouse lung confers protection against hyperoxic acute lung injury." in: Free radical biology & medicine, Vol. 84, pp. 196-205, 2015

Dey, Dutta, Sarkar, Chaudhuri: "Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice." in: Chemico-biological interactions, Vol. 235, pp. 37-46, 2015

Ling, Liu, Cheng, Luo, Yuan, Li, Xiang: "Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea." in: BioMed research international, Vol. 2015, pp. 582048, 2015

et al.: "Erratum: Borderud SP, Li Y, Burkhalter JE, Sheffer CE and Ostroff JS. Electronic cigarette use among patients with cancer: Characteristics of electronic cigarette users and their smoking cessation ..." in: Cancer, Vol. 121, Issue 5, pp. 800, 2015

Images

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details Handling ProductDetails: References for Tumor Necrosis Factor Kit (ABIN625182) back to top
Supplier Images
ELISA image for Tumor Necrosis Factor (TNF) ELISA Kit (ABIN625182) Tumor Necrosis Factor (TNF) ELISA Kit