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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||51 Antibodies||58 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies||10 Antibodies|
|Immunochromatography (IC)||3 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||38 Antibodies||38 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||55 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||30 Antibodies|
|Western Blotting (WB)||89 Antibodies|
|Antigen||BCL2-Associated X Protein (BAX) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BAX antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-BAX AntibodyTarget Details BAX Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Bax-α/β/δ/ε/σ protein (region surrounding Ser4).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details BAXProduct Details anti-BAX Antibody Application Details Handling Images back to top
|Alternative Name||Bax (BAX Antibody Abstract)|
|Background||The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4|
|Molecular Weight||approx. 21 kDa|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BAX Antibody Target Details BAX Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAX Antibody Target Details BAX Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-BAX Antibody Target Details BAX Application Details Handling back to top