BAX antibody (AA 3-16)

Catalog No. ABIN6940472

The Mouse Monoclonal anti-BAX antibody is suitable to detect BAX in samples from Human and Monkey. It has been validated for WB, IHC, IF and FACS.

Quick Overview for BAX antibody (AA 3-16) (ABIN6940472)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2D2

Product Details anti-BAX Antibody

Binding Specificity: AA 3-16

Purpose: Bax (Apoptosis Marker) Antibody

Specificity: Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX

Background:

Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

A-549 cells, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma., Jurkat, K562

Molecular Weight: 21kDa

Gene ID: 581, 624291

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response