anti-Human CDKN1A antibody for Immunofluorescence

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Epitope
AA 105-140, pSer130
50
39
27
20
18
18
18
18
15
15
15
15
13
13
12
12
9
8
7
6
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
535
186
127
30
23
10
6
5
4
2
1
1
1
1
1
1
1
1
Host
Rabbit
309
268
2
1
Clonality
Polyclonal
Conjugate
This CDKN1A antibody is un-conjugated
30
29
29
22
15
15
13
13
9
9
7
7
7
7
7
7
7
7
7
6
6
6
6
4
4
4
4
4
4
Application
Immunofluorescence (IF), Western Blotting (WB)
330
213
173
167
136
100
67
63
53
33
30
13
7
6
6
2
1
1
Options
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Catalog No. ABIN653259
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.326117 ABIN560309 IF PLA ELISA WB Mouse IgG1 kappa AA 65-164, partial Log in to see 2F1 2
12.326117 ABIN2854752 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
12.326117 ABIN1871748 IF IHC WB Rabbit IgG Log in to see Polyclonal
12.326117 ABIN4342320 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
12.326117 ABIN4342351 ICC IF IP WB Mouse IgG2a Center Log in to see 1032
12.326117 ABIN4236499 ICC IF WB Mouse IgG2b Center Log in to see 8611
12.326117 ABIN5078557 ICC IF Rabbit IgG Log in to see Polyclonal
12.326117 ABIN2404248 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN657391 IF IHC (p) WB Rabbit Ig AA 117-146, C-Term Log in to see Polyclonal 1
1 ABIN391763 FACS IF IHC (p) WB Rabbit Ig AA 134-164, C-Term Log in to see Polyclonal 5
1 ABIN4342331 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see WA-1 (HJ21) 10
1 ABIN4903234 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN385336 IF IHC (p) IP Mouse IgG2a Log in to see DCS-60-2
1 ABIN4342366 FACS IF IHC (p) Mouse IgG Log in to see CIP1-823-DCS-60-2
1 ABIN4342354 FACS IF IHC (p) SimWes WB Mouse IgG2a kappa Log in to see DCS-60-2
1 ABIN4342356 FACS IF IHC (p) WB Mouse IgG2a kappa Log in to see SPM306
1 ABIN4342358 FACS IF IHC (p) WB Mouse IgG2a kappa Log in to see SPM306
1 ABIN4342357 FACS IF IHC (p) WB Mouse IgG2a kappa Log in to see SPM306
1 ABIN4342359 FACS IF IHC (p) WB Mouse IgG2a kappa Log in to see CIP1-823
1 ABIN4342365 FACS IF IHC (p) Mouse IgG Log in to see CIP1-823-DCS-60-2

Top referenced anti-CDKN1A antibody for Immunofluorescence

Similar anti-CDKN1A Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 13 Antibodies
Dot Blot (DB) 33 Antibodies
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 173 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 101 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 134 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 210 Antibodies
Immunoprecipitation (IP) 61 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 1 Antibodies
Simple Western (SimWes) 6 Antibodies
Western Blotting (WB) 326 Antibodies

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Epitope AA 105-140, pSer130
(50), (39), (27), (20), (18), (18), (18), (18), (15), (15), (15), (15), (13), (13), (12), (12), (9), (8), (7), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(535), (186), (127), (30), (23), (10), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(309), (268), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(30), (29), (29), (22), (15), (15), (13), (13), (9), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4)
Application Immunofluorescence (IF), Western Blotting (WB)
(330), (213), (173), (167), (136), (100), (67), (63), (53), (33), (30), (13), (7), (6), (6), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN653259) Images
Specificity This P21Cip1-S130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the S130 region of human p21Cip1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This P21Cip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-140 AA from human P21Cip1.
Clone RB08044
Isotype Ig

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN653259) Images back to top
Antigen
Alternative Name P21Cip1 (CDKN1A Antibody Abstract)
Background This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Synonyms: Cyclin-dependent kinase inhibitor 1, p21, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1,
Pathways: Hepatitis C, Pathways in cancer, p53 signaling pathway, Prostate cancer, Melanoma, Bladder cancer, Cell cycle, ErbB signaling pathway, Chronic myeloid leukemia, Glioma
Molecular Weight 17988 Da
Gene ID 1026
UniProt P38936
Research Area Apoptosis/Necrosis, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling ProductDetails: References for anti-CDKN1A antibody (ABIN653259) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details ProductDetails: References for anti-CDKN1A antibody (ABIN653259) Images back to top
Format Liquid
Concentration 0.478 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-CDKN1A antibody (ABIN653259)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Chen, Turner, McCarthy, Scaltriti, Bettuzzi, Yeatman: "Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent." in: Cancer research, Vol. 64, Issue 20, pp. 7412-9, 2004

Sieburg, Tripp, Ma, Feuer: "Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 tax oncoproteins modulate cell cycle progression and apoptosis." in: Journal of virology, Vol. 78, Issue 19, pp. 10399-409, 2004

Giraud, Hurlstone, Avril, Coqueret: "Implication of BRG1 and cdk9 in the STAT3-mediated activation of the p21waf1 gene." in: Oncogene, Vol. 23, Issue 44, pp. 7391-8, 2004

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN653259) back to top
Supplier Images
Western Blotting (WB) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (AA 105-140) (ABIN653259) Western blot analysis of P21Cip1-S130 antibody (ABIN653259) in Hela cell line lysates...
Immunofluorescence (IF) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (AA 105-140) (ABIN653259) Confocal immunofluorescent analysis of P21 Antibody (WAF1) (ABIN653259) with HepG2 ce...