anti-Human CDKN1A antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Reactivity
Human
580
184
124
30
23
9
6
5
4
2
1
1
1
1
1
1
1
1
Host
Mouse
311
310
2
1
Clonality
Monoclonal
Conjugate
This CDKN1A antibody is un-conjugated
33
32
32
23
16
16
15
15
12
12
10
10
10
10
10
10
10
8
8
6
6
6
6
4
4
4
4
4
4
Application
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
356
224
186
166
151
140
67
60
59
53
29
14
7
6
6
2
1
Options
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Catalog No. ABIN966329
$ 206.25
Plus shipping costs $45.00

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Reactivity Human
(580), (184), (124), (30), (23), (9), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(311), (310), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(33), (32), (32), (23), (16), (16), (15), (15), (12), (12), (10), (10), (10), (10), (10), (10), (10), (8), (8), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
(356), (224), (186), (166), (151), (140), (67), (60), (59), (53), (29), (14), (7), (6), (6), (2), (1)
Pubmed 3 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN966329) Images
Specificity This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).
Purification Concentrated.
Immunogen Human p21 protein.
Clone B459
Isotype IgG1

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A antibody (ABIN966329) Images back to top
Antigen
Alternative Name WAF 1/p21 Protein (CDKN1A Antibody Abstract)
Molecular Weight 21 kDa
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A antibody (ABIN966329) Images back to top
Application Notes Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma.

Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A antibody (ABIN966329) Images back to top
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CDKN1A antibody (ABIN966329)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Product cited in:

Nakanishi, Robetorye, Adami, Pereira-Smith, Smith: "Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, 1995

Nakanishi, Robetorye, Pereira-Smith, Smith: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, 1995

Background publications

el-Deiry, Tokino, Velculescu, Levy, Parsons, Trent, Lin, Mercer, Kinzler, Vogelstein: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993