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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||2 Antibodies||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||13 Antibodies||4 Antibodies||4 Antibodies|
|Simple Western (SimWes)||6 Antibodies|
|Western Blotting (WB)||315 Antibodies||72 Antibodies||63 Antibodies|
|RNA Interference (RNAi)||1 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||59 Antibodies||24 Antibodies||32 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||201 Antibodies||64 Antibodies||67 Antibodies|
|Antigen||Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CDKN1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-CDKN1A AntibodyTarget Details CDKN1A Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p21 Cip1 protein. (region surrounding Gly139)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details CDKN1AProduct Details anti-CDKN1A Antibody Application Details Handling Images back to top
|Alternative Name||CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)|
|Background||It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1|
|Molecular Weight||approx. 21.0 kDa|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C|
Application DetailsProduct Details anti-CDKN1A Antibody Target Details CDKN1A Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling back to top