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anti-Human p300 antibody for Enzyme Immunoassay

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
pSer89
54
35
18
16
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
221
72
60
14
3
2
2
2
2
Host
Rabbit
155
71
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
10
10
8
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
144
94
64
57
43
40
38
24
13
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN358187
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN358269 EIA Rabbit Ig pSer1834 Log in to see Polyclonal 2
0.0008833851 ABIN782395 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2
0.0008833851 ABIN782394 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1B1
0.0008833851 ABIN116526 EMSA EIA IP WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-p300 antibody for Enzyme Immunoassay

Similar anti-p300 Antibodies

Application / Reactivity Human
ChIP DNA-Sequencing (ChIPSeq) 2 Antibodies
Chromatin Immunoprecipitation (ChIP) 6 Antibodies
Dot Blot (DB) 57 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 91 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 65 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 154 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope pSer89
(54), (35), (18), (16), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(221), (72), (60), (14), (3), (2), (2), (2), (2)
Host Rabbit
(155), (71)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(10), (10), (8), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(144), (94), (64), (57), (43), (40), (38), (24), (13), (4), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN358187) Images
Specificity This antibody detects EP300 pSer89.
Purification Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
Isotype Ig

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN358187) Images back to top
Antigen
Alternative Name EP300 / P300 (EP300 Antibody Abstract)
Background EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.Synonyms: E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT
Molecular Weight 264144 Da
Gene ID 2033, 9606
UniProt Q09472
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN358187) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN358187) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-p300 Antibody (ABIN358187)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Background publications

Finlan, Hupp: "The N-terminal interferon-binding domain (IBiD) homology domain of p300 binds to peptides with homology to the p53 transactivation domain." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 49395-405, 2004

Dornan, Eckert, Wallace, Shimizu, Ramsay, Hupp, Ball: "Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 10083-98, 2004

Jin, Jeon, Li, Lee, Choi, Kim, Lee, Bae: "Transforming growth factor-beta stimulates p300-dependent RUNX3 acetylation, which inhibits ubiquitination-mediated degradation." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29409-17, 2004

Kung, Zabludoff, France, Freedman, Tanner, Vieira, Cornell-Kennon, Lee, Wang, Wang, Memmert, Naegeli, Petersen, Eck, Bair, Wood, Livingston: "Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway." in: Cancer cell, Vol. 6, Issue 1, pp. 33-43, 2004

Chen, Halappanavar, St-Germain, Tsang, Li: "Role of Akt/protein kinase B in the activity of transcriptional coactivator p300." in: Cellular and molecular life sciences : CMLS, Vol. 61, Issue 13, pp. 1675-83, 2004

Images

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Supplier Images
 image for anti-p300 antibody (E1A Binding Protein P300) (pSer89) (ABIN358187) anti-E1A Binding Protein P300 (EP300) (pSer89) antibody
 image for anti-p300 antibody (E1A Binding Protein P300) (pSer89) (ABIN358187) anti-E1A Binding Protein P300 (EP300) (pSer89) antibody (Image 2)