anti-Human p300 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • E1A-associated protein p300
  • EP300
  • ep300
  • Ep300
Epitope
AA 1000-1050
56
53
38
18
16
11
6
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
268
126
115
39
37
14
3
3
3
3
2
Host
Rabbit
164
111
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
188
98
82
72
54
51
48
46
28
9
5
2
2
2
2
1
1
1
1
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Catalog No. ABIN152981
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.588177 ABIN800613 IF (p) IHC (p) Rabbit IgG pSer1834 Log in to see Polyclonal
13.588177 ABIN498122 IHC (p) WB Rabbit Log in to see Polyclonal
13.588177 ABIN4328393 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
13.588177 ABIN4328394 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN358187 EIA IHC (p) WB Rabbit Ig pSer89 Log in to see Polyclonal 5
1 ABIN389618 DB IHC (p) Rabbit Ig pSer89 Log in to see Polyclonal 2
1 ABIN3042372 IHC (p) WB Rabbit IgG AA 2065-2414 Log in to see Polyclonal
1 ABIN389698 DB IHC (p) Rabbit Ig pSer1834 Log in to see Polyclonal 1
1 ABIN782395 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2
1 ABIN560748 IF IHC (p) ELISA WB Mouse IgG1 AA 731-830, partial Log in to see 1B1 1
1 ABIN782394 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1B1
1 ABIN3188089 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN272047 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN790522 IF IHC (p) ELISA WB Mouse IgG1 AA 731-831 Log in to see 1B1
1 ABIN790523 IF IHC (p) ELISA WB Mouse IgG1 AA 731-831 Log in to see 1D2
1 ABIN5552531 IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN2879000 IHC (p) ELISA WB Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN2620422 IHC (p) WB Rabbit IgG AA 2065-2414 Log in to see Polyclonal
1 ABIN800622 IHC (p) HRP Rabbit IgG pSer1834 Log in to see Polyclonal
1 ABIN800652 IHC (p) HRP Rabbit IgG pSer89 Log in to see Polyclonal

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 1000-1050
(56), (53), (38), (18), (16), (11), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(268), (126), (115), (39), (37), (14), (3), (3), (3), (3), (2)
Host Rabbit
(164), (111)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(188), (98), (82), (72), (54), (51), (48), (46), (28), (9), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN152981) Images
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 antibody (ABIN152981) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Gene ID 2033
UniProt Q09472
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 antibody (ABIN152981) Images back to top
Application Notes Western Blot 1:1000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:500 to 1:2000, Immunocytochemistry/Immunofluorescence 1:250 - 1:1000, Immunoprecipitation 1-4 μg/mg of lysate, Immunohistochemistry-Paraffin 1:500 to 1:2000Western blot and immunoprecipitation. ChIP reported successful by customer review. Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 antibody (ABIN152981) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p300 antibody (ABIN152981)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Lee, Huang, Chang, Huang, Hsieh, Chen, Chou, Chiang, Lai, Shiau: "Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions." in: Scientific reports, Vol. 6, pp. 29311, 2016 Method employed by authors: Immunohistochemistry (IHC)

Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 Method employed by authors: Western Blotting (WB)

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011

Julien, Carriere, Moreau, Roux: "mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling." in: Molecular and cellular biology, Vol. 30, Issue 4, pp. 908-21, 2010

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN152981) back to top
Supplier Images
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Western Blot: KAT3B/p300 Antibody [ABIN152981] - Nuclear (NE) or cytosolic (S100) ext...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [ABIN152981] - Human lung carcinoma.
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody (ABIN152981) Western Blot: KAT3B/p300 Antibody - Detection of Human p300 by Western Blot. Samples...