|Application / Reactivity||Human||Mouse (Murine)||Monkey||Rat (Rattus)||Mink|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||179 Antibodies||14 Antibodies||79 Antibodies||70 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||46 Antibodies||15 Antibodies||27 Antibodies||27 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||45 Antibodies||1 Antibodies||1 Antibodies||19 Antibodies|
|Antigen||E1A Binding Protein P300 (EP300) Antibodies|
|Epitope||AA 2071-2091 Alternatives|
|Reactivity||Human, Mink, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This p300 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
|2 references available|
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Product Details anti-p300 AntibodyTarget Details p300 Application Details Handling References for anti-p300 Antibody (ABIN180371) Images
|Characteristics||Synonyms: CREBBP, P300, CREB-binding protein, EP300, P300 HAT, E1A binding protein p300, Histoneacetyltransferase p300, E1A-associated protein p300|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||E1A affinity purified p300 protein. Remarks: Epitope is between aa 2071-2091.|
Target Details p300Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN180371) Images back to top
|Alternative Name||EP300 / P300 (EP300 Antibody Abstract)|
|Background||P300 is a transcriptional adapter protein which is characterized by three cysteine- and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.Synonyms: CREB-binding protein, CREBBP, E1A binding protein p300, E1A-associated protein p300, EP300, Histone acetyltransferase p300, P300, P300 HAT|
|Research Area||Transcription Factors|
|Pathways||p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size|
Application DetailsProduct Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN180371) Images back to top
Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A and Ab at 2 μg/mg proteinlysate. Immunohistology (Formalin/Paraffin): Use Ab at 1/50-1/100 for 20 Minutes at RT. Stainingof formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended Positive Control: Ls174T Cells, Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN180371) Images back to top
|Buffer||10 mM PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-p300 Antibody (ABIN180371)Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Dallas, Yaciuk, Moran: "Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes." in: Journal of virology, Vol. 71, Issue 2, pp. 1726-31, 1997
Dallas, Yaciuk, Moran: "Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP." in: Hybridoma, Vol. 16, Issue 3, pp. 273-5, 1997
ImagesProduct Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN180371) back to top