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anti-Monkey p300 antibody for Western Blotting

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
265
112
100
40
39
13
3
2
2
2
2
Host
Mouse
160
111
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
186
95
82
77
67
57
52
45
24
18
5
4
2
2
1
1
1
1
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Catalog No. ABIN967440
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN1722721 ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722719 IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW105
0.0011728023 ABIN1722723 ICC IF IP WB DyLight 650 Mouse IgG1 AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722724 ICC IF IP WB DyLight 488 Mouse IgG1 AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722725 IF IP WB HRP Mouse IgG1 AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722726 ICC IF IP WB DyLight 550 Mouse IgG1 AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722722 ICC IF IP WB Biotin Mouse IgG1 AA 1572-2371 Log in to see RW128
0.0011728023 ABIN1722727 IF IP WB Biotin Mouse IgG1 AA 1572-2371 Log in to see RW105
0.0011728023 ABIN1722728 IF IP WB DyLight 650 Mouse IgG1 AA 1572-2371 Log in to see RW105
0.0011728023 ABIN1722729 IF IP WB DyLight 488 Mouse IgG1 AA 1572-2371 Log in to see RW105
0.0011728023 ABIN1722731 IF IP WB DyLight 550 Mouse IgG1 AA 1572-2371 Log in to see RW105
0.0011728023 ABIN1722730 IF IP WB HRP Mouse IgG1 AA 1572-2371 Log in to see RW105

Top referenced anti-p300 antibody for Western Blotting

Similar anti-p300 Antibodies

Application / Reactivity Human Monkey Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 186 Antibodies 13 Antibodies 72 Antibodies 64 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 79 Antibodies 14 Antibodies 54 Antibodies 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies 1 Antibodies 16 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 68 Antibodies 9 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 1 Antibodies 2 Antibodies 2 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(265), (112), (100), (40), (39), (13), (3), (2), (2), (2), (2)
Host Mouse
(160), (111)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(186), (95), (82), (77), (67), (57), (52), (45), (24), (18), (5), (4), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN967440) Images
Brand BD Pharmingen™
Cross-Reactivity Monkey, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human p300
Clone NM11
Isotype IgG2b

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN967440) Images back to top
Antigen
Alternative Name p300 (EP300 Antibody Abstract)
Background Many serotypes of human adenoviruses (Ad) are able to transform rodent cells in culture because of functions encoded by early region adenovirus 1A (E1A) and 1B (E1B) genes. These virus early gene products bind to specific cellular proteins that normally act to restrict cell growth. The E1A gene, essential for viral replication, encodes protein products with three amino acid regions (1, 2 and 3) conserved among all known adenovirus serotypes. Regions 1 and 2 and the relatively unconserved amino terminus (N) are E1A sequences required for cell growth-regulating functions. The region 2 site binds the retinoblastoma (Rb) and Rb related proteins, p107 and p130. The amino terminal site binds p300, a large cellular DNA binding protein. p300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells. A phosphatase sensitive form with decreased mobility has been identified in M-phase enriched cell populations. Evidence suggests that p300 plays a role in cellular transcription mechanisms. It has sequence-specific DNA binding activity with a consensus DNA binding sequence similar to enhancer elements targeted by the E1A repressor function. The presence of p300 and p300-associated proteins in complexes with the TATA binding protein suggests that p300 has specific interactions with basal transcription mechanisms. Through its association with the TATA regions, p300 may also play a role in the ability of E1A to activate the hsp70 promoter dependent on specific TATA box sequences. p300 migrates at a reduced molecular weight at ~300 kD. Clone NM11 recognizes human p300 a cellular E1A binding protein p300. Specifically, it has been shown to recognize p300 from human, monkey, mouse and rat cell lysates. NM11 will also coprecipitate E1A in association with p300 when E1A is present. Affinity purified p300 was used as immunogen. p300 was affinity purified from 293 human kidney cells (adenovirus type 5 [Ad5] transformed cell line) by co-immunoprecipitation using an E1A-specific monoclonal antibody (M73), which was covalently linked to protein A-Sepharose beads.
Molecular Weight 300 kDa
Research Area Transcription Factors
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN967440) Images back to top
Application Notes Applications include Western blot and immunoprecipitation. Clone NMII is routinely tested by western blot analysis using the transfer protocol for high molecular weight proteins. Ad5 transformed cell lines including 293 cells (ATCC CRL 1573) are suggested as positive controls.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN967440) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-p300 Antibody (ABIN967440)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Wang, Yaciuk, Ricciardi, Green, Yokoyama, Moran: "The E1A products of oncogenic adenovirus serotype 12 include amino-terminally modified forms able to bind the retinoblastoma protein but not p300." in: Journal of virology, Vol. 67, Issue 8, pp. 4804-13, 1993

Rikitake, Moran: "DNA-binding properties of the E1A-associated 300-kilodalton protein." in: Molecular and cellular biology, Vol. 12, Issue 6, pp. 2826-36, 1992

Background publications

Yaciuk, Moran: "Analysis with specific polyclonal antiserum indicates that the E1A-associated 300-kDa product is a stable nuclear phosphoprotein that undergoes cell cycle phase-specific modification." in: Molecular and cellular biology, Vol. 11, Issue 11, pp. 5389-97, 1991

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN967440) back to top
Supplier Images
 image for anti-p300 antibody (E1A Binding Protein P300) (ABIN967440) Immunoprecipitation of p300 and coprecipitation of E1A with NM11 (ABIN967440). NM11 i...
 image for anti-p300 antibody (E1A Binding Protein P300) (ABIN967440) anti-E1A Binding Protein P300 (EP300) antibody (Image 2)
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (ABIN967440) anti-E1A Binding Protein P300 (EP300) antibody (Image 3)