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anti-Cow (Bovine) p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 20-25
164
110
88
64
64
63
54
51
51
50
48
46
41
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Horse (Equine), Cat (Feline), Human, Sheep (Ovine)
2048
381
310
124
101
93
56
54
27
23
19
16
13
11
9
9
5
4
4
3
2
2
2
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is conjugated to PE
66
61
47
43
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Flow Cytometry (FACS)
1658
717
558
488
437
428
396
237
196
156
53
39
35
17
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN120055
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010485112 ABIN120053 FACS FITC Mouse IgG2a AA 20-25 Log in to see DO-1 2
0.0010485112 ABIN120052 FACS Biotin Mouse IgG2a AA 20-25 Log in to see DO-1 2
0.0010485112 ABIN255322 FACS FITC Mouse IgG2a Log in to see DO-1
0.0010485112 ABIN2476027 FACS Biotin Mouse IgG2a AA 20-25 Log in to see DO-1 4
0.0010485112 ABIN2476028 FACS FITC Mouse IgG2a AA 20-25 Log in to see DO-1 4
0.0010485112 ABIN2476029 FACS PE Mouse IgG2a AA 20-25 Log in to see DO-1 4
0.0010485112 ABIN4342817 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0010485112 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0010485112 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0010485112 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342835 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342836 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342837 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342838 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342839 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342841 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010485112 ABIN4342842 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2b kappa AA 37-45 Log in to see SPM590

Top referenced anti-p53 antibody for Flow Cytometry

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Application / Reactivity Human Cat (Feline) Cow (Bovine) Horse (Equine) Sheep (Ovine)
Western Blotting (WB) 1572 Antibodies 2 Antibodies 87 Antibodies 2 Antibodies 2 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 402 Antibodies 2 Antibodies 41 Antibodies 2 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(164), (110), (88), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Horse (Equine), Cat (Feline), Human, Sheep (Ovine)
(2048), (381), (310), (124), (101), (93), (56), (54), (27), (23), (19), (16), (13), (11), (9), (9), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is conjugated to PE
(66), (61), (47), (43), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Flow Cytometry (FACS)
(1658), (717), (558), (488), (437), (428), (396), (237), (196), (156), (53), (39), (35), (17), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN120055) Images
Specificity This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Bovine, Cat and Green Monkey.
Purification Affinity chromatography on Protein G
Immunogen Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Clone DO-1
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN120055) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN120055) Images back to top
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN120055) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose
Preservative Sodium azide
Precaution of Use Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN120055)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Sironi, Riccaboni, Mertel, Cammarata, Brooks: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992