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anti-Cow (Bovine) p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 20-25
152
99
83
68
64
64
55
54
54
51
49
45
43
41
32
30
29
28
24
23
23
21
20
19
17
17
15
15
15
15
15
14
11
10
9
9
9
8
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Horse (Equine), Cat (Feline), Human, Sheep (Ovine)
2131
480
395
169
126
94
68
64
34
23
18
17
16
15
14
10
6
4
4
4
4
3
3
2
2
1
Host
Mouse
1253
968
7
3
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is conjugated to PE
71
68
56
46
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Flow Cytometry (FACS)
1720
716
636
580
527
465
400
265
251
179
55
41
32
31
18
17
15
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120055
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120053 FACS FITC Mouse IgG2a AA 20-25 Log in to see DO-1 2
1 ABIN120052 FACS Biotin Mouse IgG2a AA 20-25 Log in to see DO-1 2
1 ABIN610212 FACS IHC WB PE Mouse IgG1, kappa Log in to see PAb240 3
1 ABIN610211 FACS IHC WB FITC Mouse IgG1, kappa Log in to see PAb240 3
1 ABIN610209 FACS IHC WB Mouse IgG1, kappa Log in to see PAb240 1
1 ABIN610210 FACS IHC WB Biotin Mouse IgG1, kappa Log in to see PAb240 1
1 ABIN3026348 FACS IF IHC WB Mouse IgG2b, kappa AA 20-25, N-Term Log in to see DO-7
1 ABIN385160 FACS GS IF IHC (p) IP WB Mouse IgG2a AA 11-25 Log in to see DO-1
1 ABIN3026317 FACS IF IHC WB Mouse IgG2a Log in to see BP53-12-DO-7
1 ABIN385146 FACS IF IHC (p) IP WB Mouse IgG2b, kappa Log in to see DO-7
1 ABIN576408 FACS PE Mouse IgG2a AA 20-25 Log in to see
1 ABIN206201 FACS Biotin Mouse IgG2a AA 20-25 Log in to see
1 ABIN596898 FACS FITC Mouse IgG2a Log in to see DO-1
1 ABIN4342845 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342841 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342842 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342855 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342849 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342853 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7

Top referenced anti-p53 antibody for Flow Cytometry

Similar anti-p53 Antibodies

Application / Reactivity Human Cow (Bovine) Cat (Feline) Horse (Equine) Sheep (Ovine)
Immunofluorescence (fixed cells) (IF/ICC) 28 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies 6 Antibodies
Simple Western (SimWes) 9 Antibodies 3 Antibodies
Western Blotting (WB) 1638 Antibodies 3 Antibodies 98 Antibodies 4 Antibodies 5 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 55 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(152), (99), (83), (68), (64), (64), (55), (54), (54), (51), (49), (45), (43), (41), (32), (30), (29), (28), (24), (23), (23), (21), (20), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Horse (Equine), Cat (Feline), Human, Sheep (Ovine)
(2131), (480), (395), (169), (126), (94), (68), (64), (34), (23), (18), (17), (16), (15), (14), (10), (6), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1253), (968), (7), (3), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is conjugated to PE
(71), (68), (56), (46), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS)
(1720), (716), (636), (580), (527), (465), (400), (265), (251), (179), (55), (41), (32), (31), (18), (17), (15), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN120055) Images
Specificity This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Bovine, Cat and Green Monkey.
Purification Affinity chromatography on Protein G
Immunogen Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Clone DO-1
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN120055) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN120055) Images back to top
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN120055) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose
Preservative Sodium azide
Precaution of Use Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN120055)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Sironi, Riccaboni, Mertel, Cammarata, Brooks: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992