anti-Cow (Bovine) p53 antibody for Intracellular Staining

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Reactivity
Cow (Bovine), Human, Monkey
2329
514
419
185
126
94
85
82
34
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1322
1112
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
78
76
63
53
45
44
30
30
25
25
25
24
24
24
24
23
23
23
21
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
1856
746
719
715
614
596
423
340
299
179
72
41
32
31
18
17
15
15
13
9
8
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN967458
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Top referenced anti-p53 antibody for Intracellular Staining

Similar anti-p53 Antibodies

Application / Reactivity Monkey Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 41 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 97 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 43 Antibodies
Immunofluorescence (IF) 108 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 61 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 147 Antibodies
Affinity Purification (AP) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Cow (Bovine), Human, Monkey
(2329), (514), (419), (185), (126), (94), (85), (82), (34), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1322), (1112), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(78), (76), (63), (53), (45), (44), (30), (30), (25), (25), (25), (24), (24), (24), (24), (23), (23), (23), (21), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
(1856), (746), (719), (715), (614), (596), (423), (340), (299), (179), (72), (41), (32), (31), (18), (17), (15), (15), (13), (9), (8), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN967458) Images
Brand BD Pharmingen™
Cross-Reactivity Monkey, Cow (Bovine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Recombinant p53 protein
Clone DO
Isotype IgG2b

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN967458) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. The DO-7 antibody recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies (mAbs) in normal tissues.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN967458) Images back to top
Application Notes Positive control cell lines include SK-BR-3 breast carcinoma cells (ATCC HTB-30), A431 epidermal carcinoma cells (ATCC CRL-1555) and CEM human leukemia cells (ATCC CCL-119). MCF-7 breast carcinoma cells (ATCC HTB-22) are suggested as a negative control. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN967458) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-p53 antibody (ABIN967458)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Bonsing, Corver, Gorsira, van Vliet, Oud, Cornelisse, Fleuren: "Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry." in: Cytometry, Vol. 28, Issue 1, pp. 11-24, 1997

Product cited in:

Baas, Mulder, Offerhaus, Vogelstein, Hamilton: "An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms." in: The Journal of pathology, Vol. 172, Issue 1, pp. 5-12, 1994

Cripps, Purdie, Carder, White, Komine, Bird, Wyllie: "A study of stabilisation of p53 protein versus point mutation in colorectal carcinoma." in: Oncogene, Vol. 9, Issue 9, pp. 2739-43, 1994

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Lambkin, Mothersill, Kelehan: "Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection." in: The Journal of pathology, Vol. 172, Issue 1, pp. 13-8, 1994

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (ABIN967458) Anti-human p53, ABIN967458. Formalin-fixed, paraffin-embedded tissue section of human...
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (ABIN967458) Profile of permeabilized CEM human leukemia cells analyzed on a FACScan™ (BDIS, San J...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN967458) Western blot analysis of p53 in CEM human leukemia cell lysates. Lane 1, clone DO-7 (...