|Application / Reactivity||Monkey||Human|
|ELISA||22 Antibodies||545 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||26 Antibodies|
|Immunochromatography (IC)||7 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||42 Antibodies|
|Immunohistochemistry (IHC)||47 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Western Blotting (WB)||86 Antibodies|
|Affinity Purification (AP)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||10 Antibodies|
|Dot Blot (DB)||31 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Reactivity||Cow (Bovine), Human, Monkey Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967458) Images
|Cross-Reactivity||Monkey, Cow (Bovine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Recombinant p53 protein|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN967458) Images back to top
|Alternative Name||p53 (TP53 Antibody Abstract)|
|Background||P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. The DO-7 antibody recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies (mAbs) in normal tissues.|
|Molecular Weight||53 kDa|
|Research Area||Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN967458) Images back to top
|Application Notes||Positive control cell lines include SK-BR-3 breast carcinoma cells (ATCC HTB-30), A431 epidermal carcinoma cells (ATCC CRL-1555) and CEM human leukemia cells (ATCC CCL-119). MCF-7 breast carcinoma cells (ATCC HTB-22) are suggested as a negative control. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN967458) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-p53 Antibody (ABIN967458)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
|Product cited in:||
Cripps, Purdie, Carder et al.: "A study of stabilisation of p53 protein versus point mutation in colorectal carcinoma." in: Oncogene, Vol. 9, Issue 9, pp. 2739-43, 1994 (PubMed).
Baas, Mulder, Offerhaus et al.: "An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms." in: The Journal of pathology, Vol. 172, Issue 1, pp. 5-12, 1994 (PubMed).
Lambkin, Mothersill, Kelehan: "Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection." in: The Journal of pathology, Vol. 172, Issue 1, pp. 13-8, 1994 (PubMed).
Jacquemier, Molès, Penault-Llorca et al.: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994 (PubMed).
Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991 (PubMed).
Vojt?sek, Bártek, Midgley et al.: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992 (PubMed).
Bonsing, Corver, Gorsira et al.: "Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry." in: Cytometry, Vol. 28, Issue 1, pp. 11-24, 1997 (PubMed).
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