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anti-Cow (Bovine) p53 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Reactivity
Cow (Bovine), Human
2048
381
310
124
101
93
56
54
27
23
19
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
1657
717
558
488
437
427
397
236
196
156
53
39
35
17
16
13
12
11
11
9
8
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN967457
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN967458 IHC (f) IHC (fro) ICS IP WB Mouse IgG2b Log in to see DO 7

Top referenced anti-p53 antibody for Immunohistochemistry (Formalin-fixed Sections)

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Application / Reactivity Human
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ELISA (Capture) 2 Antibodies
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Enzyme Immunoassay (EIA) 17 Antibodies
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Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 429 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Cow (Bovine), Human
(2048), (381), (310), (124), (101), (93), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(1657), (717), (558), (488), (437), (427), (397), (236), (196), (156), (53), (39), (35), (17), (16), (13), (12), (11), (11), (9), (8), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967457) Images
Brand BD Pharmingen™
Cross-Reactivity Cow (Bovine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human p53
Clone DO
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN967457) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. DO-1 reacts with human wild-type and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-1 recognizes an epitope between amino acids 1 and 45 of all known forms of human p53. Human recombinant p53 protein was used as immunogen.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN967457) Images back to top
Application Notes Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651), SK-BR-3 human breast carcinoma cells (ATCC HTB-30), and A431 human vulval carcinoma cells (ATCC CRL-1555). Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach or breast tissue.
Comment

Related Products: ABIN967389, ABIN968533

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN967457) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-p53 Antibody (ABIN967457)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Girinsky, Koumenis, Graeber, Peehl, Giaccia: "Attenuated response of p53 and p21 in primary cultures of human prostatic epithelial cells exposed to DNA-damaging agents." in: Cancer research, Vol. 55, Issue 17, pp. 3726-31, 1995

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Bártek, Bártková, Lukás, Stasková, Vojt?sek, Lane: "Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies." in: The Journal of pathology, Vol. 169, Issue 1, pp. 27-34, 1993

Product cited in:

Friedrichs, Gluba, Eidtmann, Jonat: "Overexpression of p53 and prognosis in breast cancer." in: Cancer, Vol. 72, Issue 12, pp. 3641-7, 1994

Xerri, Bouabdallah, Camerlo, Hassoun: "Expression of the p53 gene in Hodgkin's disease: dissociation between immunohistochemistry and clinicopathological data." in: Human pathology, Vol. 25, Issue 5, pp. 449-54, 1994

Bartkova, Bartek, Vojtesek, Lukas, Rejthar, Kovarik, Millis, Lane, Barnes: "Immunochemical analysis of the p53 oncoprotein in matched primary and metastatic human tumours." in: European journal of cancer (Oxford, England : 1990), Vol. 29A, Issue 6, pp. 881-6, 1993

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Images

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Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN967457) Western blot analysis of p53. Lysate from COS-7 SV40 transformed monkey kidney cells ...
 image for anti-p53 antibody (Tumor Protein P53) (ABIN967457) anti-Tumor Protein P53 (TP53) antibody (Image 2)
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN967457) anti-Tumor Protein P53 (TP53) antibody (Image 3)