anti-Cynomolgus p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 1-393
175
125
91
84
74
71
70
62
59
55
50
49
48
45
42
38
32
31
28
24
22
20
17
16
15
15
15
15
15
15
13
11
10
10
10
9
9
8
8
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cynomolgus
2388
517
424
186
127
94
85
82
35
23
20
18
17
16
15
14
5
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1365
1133
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
46
45
31
31
26
25
25
25
25
25
25
24
24
24
22
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
ELISA, ELISA (Detection), Western Blotting (WB)
1915
767
747
733
626
615
432
346
314
179
72
41
32
31
21
17
17
15
13
9
9
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2001729
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9488602 ABIN2001733 ELISA IP WB Rabbit IgG AA 1-393 Log in to see 014

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 1-393
(175), (125), (91), (84), (74), (71), (70), (62), (59), (55), (50), (49), (48), (45), (42), (38), (32), (31), (28), (24), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (13), (11), (10), (10), (10), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cynomolgus
(2388), (517), (424), (186), (127), (94), (85), (82), (35), (23), (20), (18), (17), (16), (15), (14), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1365), (1133), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (22), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application ELISA, ELISA (Detection), Western Blotting (WB)
(1915), (767), (747), (733), (626), (615), (432), (346), (314), (179), (72), (41), (32), (31), (21), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2001729) Images
Specificity Cynomolgus p53 / TP53
No cross-reactivity in ELISA with
E.coli cell lysate
No Cross-Reactivity Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus p53 / TP53 (rC p53 / TP53, ABIN2009672 E3U906, Met 1-Asp 393,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Cynomolgus p53 / TP53 protein (ABIN2009672)
Clone 06
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2001729) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background P53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53- binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.
UniProt E3U906
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN2001729) Images back to top
Application Notes WB: 10-50 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Cynomolgus P53. The detection limit for Cynomolgus P53 is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN2001729) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-p53 antibody (ABIN2001729)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Kurzhals, Titen, Xie, Golic: "Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss." in: PLoS genetics, Vol. 7, Issue 6, pp. e1002103, 2011

Kurzhals, Titen, Xie, Golic: "Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss." in: PLoS genetics, Vol. 7, Issue 6, pp. e1002103, 2011

Pardi, Vámos, Ujfaludi, Komonyi, Bodai, Boros: "In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53." in: Acta biologica Hungarica, Vol. 62, Issue 4, pp. 397-412, 2011

Wells, Johnston: "Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53." in: Developmental biology, Vol. 361, Issue 2, pp. 263-76, 2011

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN2001729) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 1-393) antibody (ABIN2001729) anti-Tumor Protein P53 (TP53) (AA 1-393) antibody