anti-Horse (Equine) p53 antibody for Immunohistochemistry (Frozen Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 20-25
175
125
91
83
74
71
70
62
59
55
50
49
48
45
42
38
32
31
28
24
22
20
17
16
15
15
15
15
15
15
14
11
10
10
10
9
9
8
8
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
2387
517
424
186
126
94
85
82
35
23
19
18
17
16
14
13
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1365
1133
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
46
45
31
31
26
25
25
25
25
25
25
24
24
24
22
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1915
768
747
732
626
615
431
346
313
179
72
41
32
31
21
17
16
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120054
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN2476020 ELISA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN198280 IHC (fro) IHC (p) IP ELISA WB Mouse IgG2a AA 20-25 Log in to see DO-1
1 ABIN576407 IHC (fro) IHC (p) Mouse IgG2a AA 20-25 Log in to see

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(175), (125), (91), (83), (74), (71), (70), (62), (59), (55), (50), (49), (48), (45), (42), (38), (32), (31), (28), (24), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (11), (10), (10), (10), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
(2387), (517), (424), (186), (126), (94), (85), (82), (35), (23), (19), (18), (17), (16), (14), (13), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1365), (1133), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (22), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1915), (768), (747), (732), (626), (615), (431), (346), (313), (179), (72), (41), (32), (31), (21), (17), (16), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN120054) Images
Specificity This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Bovine, Cat and Sheep.
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Clone DO-1
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN120054) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN120054) Images back to top
Application Notes Flow Cyometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25): Heat induced epitope retrieval with 0.01 M citrate buffer, pH 6.0 is recommended. Positive Control Tissue: Colon or breast carcinoma. ELISA. Western Blotting (1/1000). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN120054) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-p53 antibody (ABIN120054)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Sironi, Riccaboni, Mertel, Cammarata, Brooks: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001

Levesque, DCosta, Angelopoulou, Diamandis: "Time-resolved immunofluorometric assay of p53 protein." in: Clinical chemistry, Vol. 41, Issue 12 Pt 1, pp. 1720-9, 1996

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992