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anti-Horse (Equine) p53 antibody for Immunohistochemistry (Frozen Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 20-25
156
106
86
69
67
67
58
56
53
53
48
46
44
42
31
31
30
25
25
24
23
21
21
20
17
17
15
15
15
15
15
12
11
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
2176
441
335
121
100
89
57
55
29
19
18
16
15
9
9
7
5
4
4
4
3
3
2
2
2
Host
Mouse
1327
959
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
71
66
53
47
34
33
23
23
19
19
19
17
16
16
16
16
16
16
15
15
15
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
4
3
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1710
739
638
544
514
464
390
263
252
179
55
39
35
18
16
16
13
13
12
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN120054
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN2476020 IHC (fro) IHC (p) Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN2476022 IHC (fro) IHC (p) Mouse IgG2a AA 20-25 Log in to see DO-1 4

Top referenced anti-p53 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-p53 Antibodies

Application / Reactivity Sheep (Ovine) Human
Enzyme Immunoassay (EIA) 2 Antibodies 17 Antibodies
Flow Cytometry (FACS) 1 Antibodies 444 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 32 Antibodies
ELISA 689 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(156), (106), (86), (69), (67), (67), (58), (56), (53), (53), (48), (46), (44), (42), (31), (31), (30), (25), (25), (24), (23), (21), (21), (20), (17), (17), (15), (15), (15), (15), (15), (12), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine)
(2176), (441), (335), (121), (100), (89), (57), (55), (29), (19), (18), (16), (15), (9), (9), (7), (5), (4), (4), (4), (3), (3), (2), (2), (2)
Host Mouse
(1327), (959), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(71), (66), (53), (47), (34), (33), (23), (23), (19), (19), (19), (17), (16), (16), (16), (16), (16), (16), (15), (15), (15), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1710), (739), (638), (544), (514), (464), (390), (263), (252), (179), (55), (39), (35), (18), (16), (16), (13), (13), (12), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN120054) Images
Specificity This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
Cross-Reactivity (Details) Species reactivity (tested):Human, Horse, Bovine, Cat and Sheep.
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Clone DO-1
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN120054) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN120054) Images back to top
Application Notes Flow Cyometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25): Heat induced epitope retrieval with 0.01 M citrate buffer, pH 6.0 is recommended. Positive Control Tissue: Colon or breast carcinoma. ELISA. Western Blotting (1/1000). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN120054) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN120054)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Sironi, Riccaboni, Mertel, Cammarata, Brooks: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001

Levesque, DCosta, Angelopoulou, Diamandis: "Time-resolved immunofluorometric assay of p53 protein." in: Clinical chemistry, Vol. 41, Issue 12 Pt 1, pp. 1720-9, 1996

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992