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anti-Human p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 50-100
160
106
89
64
64
64
55
51
51
51
48
46
41
39
31
30
27
26
25
22
21
20
19
17
17
13
13
13
13
13
12
11
9
9
9
9
8
8
8
8
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2045
377
313
120
104
93
57
56
30
23
19
16
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Rabbit
1220
922
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1637
721
558
487
436
422
397
234
194
156
53
38
35
17
16
12
11
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152309
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30849937 ABIN2454550 FACS IF WB Mouse IgG1 Log in to see 5B2
0.30849937 ABIN2454591 FACS IF WB Mouse IgG1 Log in to see 2H4
0.30849937 ABIN2454666 FACS IF WB Mouse IgG1 Log in to see 2A4
0.30849937 ABIN2454669 FACS IF WB Mouse IgG1 Log in to see 1D1
0.30849937 ABIN2454617 FACS IF WB Mouse IgG1 Log in to see 5D9
0.30849937 ABIN2454485 FACS IF WB Mouse IgG1 Log in to see 2E4
0.30849937 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
0.30849937 ABIN2662823 FACS PE Mouse IgG2b Log in to see DO-7 2
0.30849937 ABIN2661434 FACS FITC Mouse IgG2b Log in to see DO-7 2
0.30849937 ABIN4342748 FACS ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see 5-00E02
0.001045506 ABIN863095 FACS IP IHC RIA ELISA WB Mouse AA 46-53 Log in to see Pab 1801 4
0.001045506 ABIN120055 FACS PE Mouse IgG2a AA 20-25 Log in to see DO-1 2
0.001045506 ABIN120052 FACS Biotin Mouse IgG2a AA 20-25 Log in to see DO-1 2
0.001045506 ABIN967409 FACS IF IP WB Mouse IgG2b Log in to see PAb 122 5
0.001045506 ABIN120053 FACS FITC Mouse IgG2a AA 20-25 Log in to see DO-1 2
0.001045506 ABIN94449 FACS FITC Mouse IgG2a AA 16-25 Log in to see BP53-12 7
0.001045506 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see PAb 1801 7
0.001045506 ABIN2482449 FACS IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse AA 46-53 Log in to see Pab 1801 1
0.001045506 ABIN2482446 FACS IP RIA IHC ELISA WB Atto 655 Mouse AA 46-53 Log in to see Pab 1801 1
0.001045506 ABIN2482452 FACS IP RIA IHC ELISA WB FITC Mouse AA 46-53 Log in to see Pab 1801 1

Top referenced anti-p53 antibody for Flow Cytometry

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Application / Reactivity Human
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Dot Blot (DB) 31 Antibodies
ELISA 670 Antibodies
ELISA (Capture) 2 Antibodies
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Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 381 Antibodies
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Immunoassay (IA) 1 Antibodies
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Immunocytochemistry (ICC) 189 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 428 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 50-100
(160), (106), (89), (64), (64), (64), (55), (51), (51), (51), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (21), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (9), (9), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2045), (377), (313), (120), (104), (93), (57), (56), (30), (23), (19), (16), (13), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Rabbit
(1220), (922), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1637), (721), (558), (487), (436), (422), (397), (234), (194), (156), (53), (38), (35), (17), (16), (12), (11), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) Images
Specificity This is specific for human p53.
Cross-Reactivity (Details) The antibody is not likely to react with the mouse ortholog owing to divergence of sequence at the site to which the epitope maps.
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Application Notes Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μg
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN152309) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate buffer, pH 7 to 8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p53 Antibody (ABIN152309)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." in: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." in: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (Sample species: Human). Further details: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." in: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." in: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...