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anti-Human p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 50-100
156
105
87
67
67
65
56
54
52
51
46
46
42
40
31
31
28
25
25
23
22
21
19
17
17
13
13
13
13
13
12
11
11
10
10
9
9
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2129
409
327
121
101
89
57
55
29
23
19
16
15
10
10
5
5
4
4
4
3
3
3
2
2
Host
Rabbit
1287
955
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
70
64
51
46
33
32
23
23
18
18
18
17
16
15
15
15
15
15
14
14
14
14
14
14
14
14
7
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1694
744
619
536
500
447
385
256
249
156
54
39
35
18
17
16
12
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152309
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.7474785 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
13.7474785 ABIN2454591 FACS IF WB Mouse IgG1 Log in to see 2H4
13.7474785 ABIN2454669 FACS IF WB Mouse IgG1 Log in to see 1D1
13.7474785 ABIN2454485 FACS IF WB Mouse IgG1 Log in to see 2E4
13.7474785 ABIN2454550 FACS IF WB Mouse IgG1 Log in to see 5B2
13.7474785 ABIN2454617 FACS IF WB Mouse IgG1 Log in to see 5D9
13.7474785 ABIN2454666 FACS IF WB Mouse IgG1 Log in to see 2A4
13.7474785 ABIN2662823 FACS PE Mouse IgG2b Log in to see DO-7 2
13.7474785 ABIN2661434 FACS FITC Mouse IgG2b Log in to see DO-7 2
13.7474785 ABIN4898072 FACS PE Mouse IgG1 pSer15 Log in to see 261352
13.7474785 ABIN4897761 FACS Allophycocyanin Mouse IgG2b AA 7-393 Log in to see 184727
13.7474785 ABIN4900612 FACS WB Mouse IgG1 pSer15 Log in to see 261352
13.7474785 ABIN4900613 FACS ICC Mouse IgG1 pSer15 Log in to see 261366
13.7474785 ABIN4342748 FACS ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see 5E2
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 19
1 ABIN863095 FACS IP IHC RIA ELISA WB Mouse AA 46-53 Log in to see Pab 1801 4
1 ABIN120055 FACS PE Mouse IgG2a AA 20-25 Log in to see DO-1 2
1 ABIN120052 FACS Biotin Mouse IgG2a AA 20-25 Log in to see DO-1 2
1 ABIN120053 FACS FITC Mouse IgG2a AA 20-25 Log in to see DO-1 2
1 ABIN967409 FACS IF IP WB Mouse IgG2b Log in to see PAb 122 5

Top referenced anti-p53 antibody for Flow Cytometry

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Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 32 Antibodies
ELISA 689 Antibodies
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Immunoassay (IA) 1 Antibodies
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Immunocytochemistry (ICC) 230 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 477 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 568 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 50-100
(156), (105), (87), (67), (67), (65), (56), (54), (52), (51), (46), (46), (42), (40), (31), (31), (28), (25), (25), (23), (22), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2129), (409), (327), (121), (101), (89), (57), (55), (29), (23), (19), (16), (15), (10), (10), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2)
Host Rabbit
(1287), (955), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(70), (64), (51), (46), (33), (32), (23), (23), (18), (18), (18), (17), (16), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1694), (744), (619), (536), (500), (447), (385), (256), (249), (156), (54), (39), (35), (18), (17), (16), (12), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) Images
Specificity This is specific for human p53.
Cross-Reactivity (Details) The antibody is not likely to react with the mouse ortholog owing to divergence of sequence at the site to which the epitope maps.
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Gene ID 7157
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Application Notes Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μgImmunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 μg/30 μg chromatin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN152309) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p53 Antibody (ABIN152309)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." in: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." in: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (Sample species: Human). Further details: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." in: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." in: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...