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anti-Human p53 antibody for Proximity Ligation Assay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 50-100
152
99
84
68
64
64
55
54
54
51
49
45
43
41
32
30
29
28
24
23
23
21
19
19
17
17
15
15
15
15
15
14
11
10
9
9
9
8
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2131
480
395
169
127
94
68
64
34
23
18
17
16
16
15
11
6
4
4
4
4
3
3
2
2
1
Host
Rabbit
1252
969
7
3
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
71
68
56
47
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1719
716
635
579
526
465
399
264
251
179
55
41
32
31
18
17
15
14
13
9
9
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN152309
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.19914 ABIN521046 IHC (p) PLA RNAi ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C11
32.19914 ABIN521045 PLA WB Rabbit AA 1-393, full length Log in to see Polyclonal
1 ABIN1943403 FACS IF IP IHC (p) PLA WB Rabbit IgG AA 50-100 Log in to see Polyclonal

Top referenced anti-p53 antibody for Proximity Ligation Assay

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 14 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 27 Antibodies
ELISA 666 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 458 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 41 Antibodies
Immunocytochemistry (ICC) 247 Antibodies
Immunodiffusion (ID) 15 Antibodies
Immunofluorescence (IF) 507 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 28 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 50-100
(152), (99), (84), (68), (64), (64), (55), (54), (54), (51), (49), (45), (43), (41), (32), (30), (29), (28), (24), (23), (23), (21), (19), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2131), (480), (395), (169), (127), (94), (68), (64), (34), (23), (18), (17), (16), (16), (15), (11), (6), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Host Rabbit
(1252), (969), (7), (3), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(71), (68), (56), (47), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1719), (716), (635), (579), (526), (465), (399), (264), (251), (179), (55), (41), (32), (31), (18), (17), (15), (14), (13), (9), (9), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) Images
Specificity This is specific for human p53.
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Gene ID 7157
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Application Notes Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μgImmunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 μg/30 μg chromatin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN152309) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p53 Antibody (ABIN152309)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." in: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." in: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (Sample species: Human). Further details: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." in: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." in: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...