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anti-Non-Human Primate p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 16-25
123
87
62
54
51
50
49
48
46
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
7
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Non-Human Primate
1760
319
269
99
34
19
13
11
11
10
10
9
5
4
4
3
3
3
3
2
2
2
1
Host
Mouse
1235
612
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1378
581
529
319
314
302
235
156
99
86
53
39
35
17
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN94448
$ 242.55
Plus shipping costs $45.00

Top referenced anti-p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-p53 Antibodies

Application / Reactivity Non-Human Primate Human
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Flow Cytometry (FACS) 1 Antibodies 220 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 1 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 11 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 16-25
(123), (87), (62), (54), (51), (50), (49), (48), (46), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Non-Human Primate
(1760), (319), (269), (99), (34), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1)
Host Mouse
(1235), (612), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1378), (581), (529), (319), (314), (302), (235), (156), (99), (86), (53), (39), (35), (17), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN94448) Images
Specificity The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells, it is frequently mutated or inactivated in many types of cancer.
Purification Purified from ascites by precipitation methods.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed full-length wild-type p53
Clone BP53-12
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN94448) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN94448) Images back to top
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL, overnight in 4 °C
Positive control: RAMOS human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (11 % separating gel).

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN94448) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-p53 Antibody (ABIN94448)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Tanigawa, Fujii, Hou: "Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells." in: Bioscience, biotechnology, and biochemistry, Vol. 72, Issue 3, pp. 797-804, 2008

Taylor, Agarwal, Agarwal, Stacey, Stark: "p53 inhibits entry into mitosis when DNA synthesis is blocked." in: Oncogene, Vol. 18, Issue 2, pp. 283-95, 1999

Taylor, DePrimo, Agarwal, Agarwal, Schönthal, Katula, Stark: "Mechanisms of G2 arrest in response to overexpression of p53." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3607-22, 1999

Agarwal, Agarwal, Taylor, Stark: "p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8493-7, 1995

Product cited in:

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, 1997

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, 1991

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN94448) back to top
Supplier Images
Confocal Microscopy (CM) image for anti-p53 antibody (Tumor Protein P53) (AA 16-25) (ABIN94448) Confocal microscopy of human HeLa cells using anti-p53 (BP53-12 ; FITC). The expressi...